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Advisor(s)
Abstract(s)
Pinewood nematode (PWN, Bursaphelenchus xylophilus) is the causal agent of pine wilt
disease (PWD), which severely affects Pinus pinaster stands in southwestern Europe. Despite the high
susceptibility of P. pinaster, individuals of selected half-sib families have shown genetic variability in
survival after PWN inoculation, indicating that breeding for resistance can be a valuable strategy
to control PWD. In this work, RNA-seq data from susceptible and resistant plants inoculated with
PWN were used for SNP discovery and analysis. A total of 186,506 SNPs were identified, of which
31 were highly differentiated between resistant and susceptible plants, including SNPs in genes
involved in cell wall lignification, a process previously linked to PWN resistance. Fifteen of these
SNPs were selected for validation through Sanger sequencing and 14 were validated. To evaluate
SNP-phenotype associations, 40 half-sib plants were genotyped for six validated SNPs. Associations
with phenotype after PWN inoculation were found for two SNPs in two different genes (MEE12
and PCMP-E91), as well as two haplotypes of HIPP41, although significance was not maintained
following Bonferroni correction. SNPs here detected may be useful for the development of molecular
markers for PWD resistance and should be further investigated in future association studies
Description
Keywords
maritime pine pine wilt disease RNA-seq single nucleotide polymorphism molecular markers
Pedagogical Context
Citation
Modesto, I.; Inácio, V.; Novikova, P.; Carrasquinho, I.; Van de Peer, Y.; Miguel, C.M. SNP Detection in Pinus pinaster Transcriptome and Association with Resistance to Pinewood Nematode. Forests 2022, 13, 946
Publisher
MDPI