Publicação
Integrated Omics analysis of pig muscle metabolism under the effects of dietary Chlorella vulgaris and exogenous enzymes
| dc.contributor.author | Coelho, Diogo | |
| dc.contributor.author | Ribeiro, David | |
| dc.contributor.author | Osório, Hugo | |
| dc.contributor.author | Almeida, André | |
| dc.contributor.author | Prates, José António Mestre | |
| dc.date.accessioned | 2022-10-20T14:08:37Z | |
| dc.date.available | 2022-10-20T14:08:37Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Monogastric feeding is dependent on costly conventional feedstuffs. Microalgae such as Chlorella vulgaris are a sustainable alternative; however, its recalcitrant cell wall hinders monogastric digestion. Carbohydrate Active Enzyme (CAZyme) supplementation is a possible solution. The objective of this work was to evaluate the effect of 5% dietary C. vulgaris (CV) and enzymatic supplementation (CV + R— Rovabio® Excel AP; CV + M—four CAZyme mix) on muscle transcriptome and proteome of finishing pigs, in an integrated approach. Control pigs increased the abundance of contractile apparatus (MYH1, MYH2, MYH4) and energy metabolism (CKMT1, NDUFS3) proteins, demonstrating increased nutrient availability. They had increased expression of SCD, characteristic of increased glucose availability, via the activation of SREBP-1c and ChREBP. CV and CV + R pigs upregulated proteolytic and apoptotic genes (BAX, DDA1), whilst increasing the abundance of glucose (UQCRFS1) and fatty acid catabolism (ACADS) proteins. CV + R pigs upregulated ACOT8 and SIRT3 genes as a response to reduced nutrient availability, maintaining energy homeostasis. The cell wall specific CAZyme mix, CV + M, was able to comparatively reduce Omics alterations in the muscle, thereby reducing endogenous nutrient catabolism compared to the CV + R and CV | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Scientific Reports, (2022) 12:16992 | pt_PT |
| dc.identifier.doi | https://doi.org/10.1038/s41598-022-21466-z | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.5/25758 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Nature | pt_PT |
| dc.relation | Linking Landscape, Environment, Agriculture and Food | |
| dc.relation | Improving the nutritional value of microalgae for feeding pigs through the use of novel enzymes | |
| dc.relation | Inclusion of Laminaria and Ulva seaweeds as a feedstuff in piglet diets: effect on animal performance and physiology | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Chlorella vulgaris | pt_PT |
| dc.subject | pig | pt_PT |
| dc.subject | enzyme | pt_PT |
| dc.subject | omics | pt_PT |
| dc.title | Integrated Omics analysis of pig muscle metabolism under the effects of dietary Chlorella vulgaris and exogenous enzymes | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Linking Landscape, Environment, Agriculture and Food | |
| oaire.awardTitle | Improving the nutritional value of microalgae for feeding pigs through the use of novel enzymes | |
| oaire.awardTitle | Inclusion of Laminaria and Ulva seaweeds as a feedstuff in piglet diets: effect on animal performance and physiology | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT-NUT%2F5931%2F2014/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04129%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F126198%2F2016/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F143992%2F2019/PT | |
| oaire.citation.title | Scientific Reports | pt_PT |
| oaire.fundingStream | 3599-PPCDT | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| person.familyName | Martinho de Almeida | |
| person.givenName | André | |
| person.identifier.ciencia-id | 1E14-92CC-B163 | |
| person.identifier.orcid | 0000-0002-7810-3988 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | 722b3039-6553-4059-8e11-d5eaf113a8cd | |
| relation.isAuthorOfPublication.latestForDiscovery | 722b3039-6553-4059-8e11-d5eaf113a8cd | |
| relation.isProjectOfPublication | 318ca810-f36e-45f6-8e63-2cfcb5488b48 | |
| relation.isProjectOfPublication | 68ef0aed-47b8-469e-819f-7175024862e9 | |
| relation.isProjectOfPublication | ebf1a0f6-fc3a-4397-bfc4-1f70db436ed0 | |
| relation.isProjectOfPublication | f9a66ffe-a149-4ad9-9b82-8ebd2159996c | |
| relation.isProjectOfPublication.latestForDiscovery | 318ca810-f36e-45f6-8e63-2cfcb5488b48 |