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Comparing biomarker responses during thermal acclimation: A lethal vs non-lethal approach in a tropical reef clownfish

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dc.contributor.authorMadeira, Carolina
dc.contributor.authorMadeira, Diana
dc.contributor.authorDiniz, Mário S.
dc.contributor.authorCabral, Henrique
dc.contributor.authorVinagre, Catarina
dc.date.accessioned2019-06-25T15:40:42Z
dc.date.available2019-06-25T15:40:42Z
dc.date.issued2017
dc.description.abstractKnowledge of thermal stress biology for most tropical fish species in reef ecosystems under climate change is still quite limited. Thus, the objective of this study was to measure the time-course changes of thermal stress biomarkers in the commercially exploited coral reef fish Amphiprion ocellaris, during a laboratory simulated event of increased temperature. Heat shock protein 70kDa (Hsp70) and total ubiquitin (Ub) were determined in the muscle (lethal method) and in the fin (non-lethal alternative method) under two temperature treatments (control - 26°C and elevated temperature - 30°C) throughout one month with weekly samplings. Results suggest that biomarker basal levels are tissue-specific and influence the degree of response under temperature exposure. Responses were highly inducible in the muscle but not in fin tissue, indicating that the latter is not reliable for monitoring purposes. Thermal stress was observed in the muscle after one week of exposure (both biomarkers increased significantly) and Ub levels then decreased, suggesting the animals were able to acclimate by maintaining high levels of Hsp70 and through an effective protein turnover. In addition, the results show that mortality rates did not differ between treatments. This indicates that A. ocellaris is capable of displaying a plastic response to elevated temperature by adjusting the protein quality control system to protect cell functions, without decreasing survival. Thus, this coral reef fish species presents a significant acclimation potential under ocean warming scenarios of +4°C. Monitoring of thermal stress through a non-lethal method, fin-clipping, although desirable proved to be inadequate for this species.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.cbpa.2016.11.018pt_PT
dc.identifier.issn1095-6433
dc.identifier.urihttp://hdl.handle.net/10451/38867
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectBiomonitoringpt_PT
dc.subjectClownfishpt_PT
dc.subjectCoral reefspt_PT
dc.subjectEcophysiologypt_PT
dc.subjectMolecular biomarkerspt_PT
dc.subjectThermal biologypt_PT
dc.titleComparing biomarker responses during thermal acclimation: A lethal vs non-lethal approach in a tropical reef clownfishpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage112pt_PT
oaire.citation.startPage104pt_PT
oaire.citation.titleComparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiologypt_PT
oaire.citation.volume204pt_PT
person.familyNameGusmão Coito Madeira
person.familyNameCabral
person.familyNameVinagre
person.givenNameSara Carolina
person.givenNameHenrique
person.givenNameCatarina
person.identifierIpgi3X4AAAAJ&hl
person.identifier138360
person.identifier.ciencia-id2419-0840-DBED
person.identifier.ciencia-idCD19-4B30-2D53
person.identifier.ciencia-id3F13-843A-791B
person.identifier.orcid0000-0003-1632-634X
person.identifier.orcid0000-0002-7646-6208
person.identifier.orcid0000-0003-2146-7948
person.identifier.ridD-5201-2011
person.identifier.scopus-author-id55345528500
person.identifier.scopus-author-id7006073782
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1e6baa2d-fde9-459a-88ec-842fa51aef11
relation.isAuthorOfPublication36de15f7-6c1c-4cce-ac65-5719d7ac4faa
relation.isAuthorOfPublication9adfabdb-a961-4c8f-9bfa-9d3dde0f94da
relation.isAuthorOfPublication.latestForDiscovery1e6baa2d-fde9-459a-88ec-842fa51aef11

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