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The expression of Helicobacter pylori tfs plasticity zone cluster is regulated by pH and adherence, and its composition is associated with differential gastric IL-8 secretion

2017Journal article Restricted access
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dc.contributor.authorSilva, Bruno
dc.contributor.authorNunes, Alexandra
dc.contributor.authorVale, Filipa
dc.contributor.authorRocha, Raquel
dc.contributor.authorGomes, João Paulo
dc.contributor.authorDias, Ricardo
dc.contributor.authorOleastro, Mónica
dc.date.accessioned2022-09-08T15:05:53Z
dc.date.available2022-09-08T15:05:53Z
dc.date.issued2017
dc.description© 2017 John Wiley & Sons Ltd.pt_PT
dc.description.abstractBackground: Helicobacter pylori virulence is associated with different clinical outcomes. The existence of an intact dupA gene from tfs4b cluster has been suggested as a predictor for duodenal ulcer development. However, the role of tfs plasticity zone clusters in the development of ulcers remains unclear. We studied several H. pylori strains to characterize the gene arrangement of tfs3 and tfs4 clusters and their impact in the inflammatory response by infected gastric cells. Methods: The genome of 14 H. pylori strains isolated from Western patients, pediatric (n=10) and adult (n=4), was fully sequenced using the Illumina platform MiSeq, in addition to eight pediatric strains previously sequenced. These strains were used to infect human gastric cells, and the secreted interleukin-8 (IL-8) was quantified by ELISA. The expression of virB2, dupA, virB8, virB10, and virB6 was assessed by quantitative PCR in adherent and nonadherent fractions of H. pylori during in vitro co-infection, at different pH values. Results: We have found that cagA-positive H. pylori strains harboring a complete tfs plasticity zone cluster significantly induce increased production of IL-8 from gastric cells. We have also found that the region spanning from virB2 to virB10 genes constitutes an operon, whose expression is increased in the adherent fraction of bacteria during infection, as well as in both adherent and nonadherent fractions at acidic conditions. Conclusions: A complete tfs plasticity zone cluster is a virulence factor that may be important for the colonization of H. pylori and to the development of severe outcomes of the infection with cagA-positive strains.pt_PT
dc.description.sponsorshipThis work was supported by the FCT-PTDC/BIM-MEC/1051/2012 grant from the Fundação para a Ciência e a Tecnologia (FCT) (to M.O.). B.S. and F.F.V. are recipients of postdoctoral fellowships (PTDC/BIM- MEC/1051/2012 and SFRH/BPD/95125/2013, respectively) from FCT, and R.R is recipient of a fellowship (BRJ-DDI/2012) from the National Institute of Health. WGS and capillary sequencing were performed at Unidade de Tecnologia e Inovação (Departamento de Genética Humana, Instituto Nacional de Saúde Dr Ricardo Jorge, Lisbon, Portugal).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHelicobacter. 2017 Aug;22(4). doi: 10.1111/hel.12390pt_PT
dc.identifier.doi10.1111/hel.12390pt_PT
dc.identifier.eissn1523-5378
dc.identifier.issn1083-4389
dc.identifier.urihttp://hdl.handle.net/10451/54390
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationFCT-PTDC/BIM-MEC/1051/2012pt_PT
dc.relationMicro-lysis: studing Helicobacter pylori phages for phage therapy
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/journal/15235378pt_PT
dc.subjectHelicobacter pyloript_PT
dc.subjectAdhesionpt_PT
dc.subjectComplete tfs clusterpt_PT
dc.subjectInterleukin-8pt_PT
dc.subjectOperonpt_PT
dc.subjectpHpt_PT
dc.titleThe expression of Helicobacter pylori tfs plasticity zone cluster is regulated by pH and adherence, and its composition is associated with differential gastric IL-8 secretionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMicro-lysis: studing Helicobacter pylori phages for phage therapy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F95125%2F2013/PT
oaire.citation.issue4pt_PT
oaire.citation.titleHelicobacterpt_PT
oaire.citation.volume22pt_PT
person.familyNameSousa Silva
person.familyNameVale
person.givenNameBruno Adriano
person.givenNameFilipa
person.identifier.ciencia-id6E10-32B5-C862
person.identifier.ciencia-id4718-00EF-4BF0
person.identifier.orcid0000-0002-1452-9779
person.identifier.orcid0000-0003-4635-0105
person.identifier.ridC-3570-2013
person.identifier.scopus-author-id57209021272
person.identifier.scopus-author-id17136133700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa43f423d-cfc5-4a4e-b713-001912a4b833
relation.isAuthorOfPublicationb2d398b3-1980-4cc0-9905-9e478bdb85a9
relation.isAuthorOfPublication.latestForDiscoverya43f423d-cfc5-4a4e-b713-001912a4b833
relation.isProjectOfPublicationa504465f-9de4-4696-808a-b41c24e01102
relation.isProjectOfPublication.latestForDiscoverya504465f-9de4-4696-808a-b41c24e01102

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