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Applications of genome editing by programmable nucleases to the metabolic engineering of secondary metabolites

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dc.contributor.authorLeitão, Ana Lúcia
dc.contributor.authorCosta, Marina C.
dc.contributor.authorEnguita, Francisco J.
dc.date.accessioned2021-12-02T16:00:18Z
dc.date.available2021-12-02T16:00:18Z
dc.date.issued2017
dc.description© 2016 Elsevier B.V. All rights reserved.pt_PT
dc.description.abstractGenome engineering is a branch of modern biotechnology composed of a cohort of protocols designed to construct and modify a genotype with the main objective of giving rise to a desired phenotype. Conceptually, genome engineering is based on the so called genome editing technologies, a group of genetic techniques that allow either to delete or to insert genetic information in a particular genomic locus. Ten years ago, genome editing tools were limited to virus-driven integration and homologous DNA recombination. However, nowadays the uprising of programmable nucleases is rapidly changing this paradigm. There are two main families of modern tools for genome editing depending on the molecule that controls the specificity of the system and drives the editor machinery to its place of action. Enzymes such as Zn-finger and TALEN nucleases are protein-driven genome editors; while CRISPR system is a nucleic acid-guided editing system. Genome editing techniques are still not widely applied for the design of new compounds with pharmacological activity, but they are starting to be considered as promising tools for rational genome manipulation in biotechnology applications. In this review we will discuss the potential applications of programmable nucleases for the metabolic engineering of secondary metabolites with biological activity.pt_PT
dc.description.sponsorshipM.C.C. was supported by a postdoctoral fellowship from Fundação para a Ciência e Tecnologia, Portugal (Ref. SFRH/BPD/65131/2009).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJournal of biotechnology, 241, 50-60pt_PT
dc.identifier.doi10.1016/j.jbiotec.2016.11.009pt_PT
dc.identifier.eissn1873-4863
dc.identifier.issn0168-1656
dc.identifier.urihttp://hdl.handle.net/10451/50257
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationE-SMART: ENGINEERING OF MIRNA TARGET SITES IN CODING TRANSCRIPTS
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/journal-of-biotechnologypt_PT
dc.subjectCRISPR-Cas9pt_PT
dc.subjectEpigenome editingpt_PT
dc.subjectHybrid metabolitespt_PT
dc.subjectSecondary metabolismpt_PT
dc.subjectTALENpt_PT
dc.titleApplications of genome editing by programmable nucleases to the metabolic engineering of secondary metabolitespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleE-SMART: ENGINEERING OF MIRNA TARGET SITES IN CODING TRANSCRIPTS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F65131%2F2009/PT
oaire.citation.endPage60pt_PT
oaire.citation.startPage50pt_PT
oaire.citation.titleJournal of Biotechnologypt_PT
oaire.citation.volume241pt_PT
person.familyNameCosta
person.familyNameEnguita
person.givenNameMarina
person.givenNameFrancisco J.
person.identifier173306
person.identifier.ciencia-id6818-7810-6CEC
person.identifier.ciencia-idB215-8D49-3218
person.identifier.orcid0000-0001-9020-7539
person.identifier.orcid0000-0002-8072-8557
person.identifier.ridO-1710-2014
person.identifier.ridA-2347-2009
person.identifier.scopus-author-id55453276400
person.identifier.scopus-author-id6602119231
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationa101dbd4-6bf5-4546-882e-e2596eddb6b4
relation.isAuthorOfPublication.latestForDiscoverya101dbd4-6bf5-4546-882e-e2596eddb6b4
relation.isProjectOfPublication404c868f-1b7d-402a-869d-09c41b4817da
relation.isProjectOfPublication.latestForDiscovery404c868f-1b7d-402a-869d-09c41b4817da

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