Repository logo
 
Publication

Preparation and Characterization of Films Based on a Natural P(3HB)/mcl-PHA Blend Obtained through the Co-culture of Cupriavidus Necator and Pseudomonas Citronellolis in Apple Pulp Waste

2020Journal article Open access
Simple item page
dc.contributor.authorRebocho, Ana Teresa
dc.contributor.authorPereira, João R.
dc.contributor.authorNeves, Luísa A.
dc.contributor.authorDelgado Alves, Vitor
dc.contributor.authorSevrin, Chantal
dc.contributor.authorGrandfils, Christian
dc.contributor.authorFreitas, Filomena
dc.contributor.authorReis, Maria A.M.
dc.date.accessioned2021-09-29T13:25:05Z
dc.date.available2021-09-29T13:25:05Z
dc.date.issued2020
dc.description.abstractThe co-culture of Cupriavidus necator DSM 428 and Pseudomonas citronellolis NRRL B-2504 was performed using apple pulp waste from the fruit processing industry as the sole carbon source to produce poly(3-hydroxybutyrate), P(3HB) and medium-chain length PHA, mcl-PHA, respectively. The polymers accumulated by both strains were extracted from the co-culture’s biomass, resulting in a natural blend that was composed of around 48 wt% P(3HB) and 52 wt% mcl-PHA, with an average molecular weight of 4.3 x 10 5 Da and a polydispersity index of 2.2. Two melting temperatures (Tm) were observed for the blend, 52 and 174 ºC, which correspond to the Tm of the mcl-PHA and P(3HB), respectively. P(3HB)/mcl-PHA blend films prepared by the solvent evaporation method had permeabilities to oxygen and carbon dioxide of 2.6 and 32 Barrer, respectively. The films were flexible and easily deformed, as demonstrated by their tensile strength at break of 1.47 +- 0.07 MPa, with a deformation of 338 +- 19% until breaking, associated with a Young modulus of 5.42 +- 1.02 MPa. This study demonstrates for the first time the feasibility of using the co-culture of C. necator and P. citronellolis strains to obtain a natural blend of P(3HB)/mcl-PHA that can be processed into films suitable for applications ranging from commodity packaging products to high-value biomaterialspt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBioengineering 2020, 7, 34pt_PT
dc.identifier.doi10.3390/bioengineering7020034pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/22102
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationUID/QUI/50006/2020pt_PT
dc.relationPEst-OE/AGR/UI0245/2020pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectapple pulp wastept_PT
dc.subjectpolyhydroxyalkanoates (PHA)pt_PT
dc.subjectco-culturept_PT
dc.subjectpolymer blendpt_PT
dc.subjectfilmspt_PT
dc.titlePreparation and Characterization of Films Based on a Natural P(3HB)/mcl-PHA Blend Obtained through the Co-culture of Cupriavidus Necator and Pseudomonas Citronellolis in Apple Pulp Wastept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157549/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/75374/PT
oaire.citation.titleBioengineeringpt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
person.familyNameDelgado Alves
person.givenNameVitor
person.identifier1916677
person.identifier.ciencia-id2E1E-6918-4DB3
person.identifier.orcid0000-0002-4117-5582
person.identifier.scopus-author-id24536716000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication8d1a8489-8789-4569-89e2-0bade23dd945
relation.isAuthorOfPublication.latestForDiscovery8d1a8489-8789-4569-89e2-0bade23dd945
relation.isProjectOfPublicationb69df505-c1cb-42d5-b901-59998ffbc4da
relation.isProjectOfPublicationa6f15a77-274e-4720-ba48-dd52f74fdb1e
relation.isProjectOfPublication.latestForDiscoveryb69df505-c1cb-42d5-b901-59998ffbc4da

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
REP-LEAF-VALVES-Preparation and Characterization of Films Based MDPI.pdf
Size:
6.56 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISA - Artigos em Revistas Internacionais