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Publicação
The TCN2 776C> G polymorphism correlates with vitamin B12 cellular delivery in healthy adult populations
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Orientador(es)
Resumo(s)
Objectives: Vitamin B12, or B12, is an essential nutrient for humans, and its deficiency is a public health problem, especially in elderly population. Around 30% of circulating total B12 levels are attached to transcobalamin II (TCN2), being referred as holotranscobalamin (holo-TC), and representing the biologically active fraction. After cellular uptake, B12 participates in the homocysteine (Hcy) metabolism. The potential influence of the described TCN2 776C> G polymorphism upon B12 intracellular delivery is a current target of
research and we aimed to investigate its biochemical significance upon a healthy adult population.
Design and methods: The TCN2 776C> G polymorphism was screened by PCR-RFLP in 122 individuals.
Concentrations of plasma total B12, holo-TC, total Hcy and folate, as well as red blood cell folate, were determined.
Results and conclusions: The studied polymorphism is common in the Portuguese population and significantly affects holo-TC but neither total B12 nor total Hcy plasma concentrations, confirming that the TCN2 776C> G genotype exerts a significant influence upon B12 cellular delivery.
Descrição
© 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
Palavras-chave
Transcobalamin polymorphism Vitamin B12 delivery Homocysteine
Contexto Educativo
Citação
Clinical Biochemistry 43(2010)645–649