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Selecting Aragonez genotypes able to outplay climate change-driven abiotic stress
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Orientador(es)
Resumo(s)
High temperatures and extreme drought are increasingly more frequent in Portugal,
which represents a strong threat to viticulture in certain regions of the country. These
multifactorial abiotic stresses are threatening viticultural areas worldwide, and the
problem can hardly be overcome only by changing cultural practices. This scenario has
raised a major challenge for plant scientists to find ways to adapt existing varieties to the
new conditions without loss of their characteristic flavors, yield, and associated varietal
character of wines. Aragonez (syn. Tempranillo) is one such variety, widely cultivated in
Portugal and Spain, with specific characteristics associated with terroir. In this context,
insight into intravarietal variability to enable its exploitation for selection becomes an
important tool to mitigate the effect of multifactorial stresses driven by climate changes.
The present work describes an innovative selection approach: selection for abiotic
stress tolerance, measured by the leaf temperature of clones under environmental
conditions of drought and extreme heat. This evaluation was complemented with values
of yield and quality characteristics of the must (pH, acidity, Brix, and anthocyanins). The
application of this methodology was done in an experimental population of 255 clones
of Tempranillo for 3 years. The genotypes were then ranked according to their level of
tolerance to abiotic stress without loss of yield/quality. To understand the differences at
the transcription level that could account for such variability, several of the most tolerant
and most sensitive genotypes were analyzed for key genes using reverse transcriptase–
quantitative polymerase chain reaction. The results enabled the selection of a group of
genotypes with increased tolerance to stress, in relation to the average of the variety,
which maintained the typical must quality of Aragonez. In parallel, several transcripts
previously acknowledged as markers for abiotic stress tolerance were identified in
several clones and are possible targets for plant breeding and genetic modification
and/or to develop screening procedures to select genotypes better adapted to the
abiotic stress driven by climate change
Descrição
Original Research
Palavras-chave
abiotic stress tolerance empirical best linear unbiased predictors (EBLUP of genotypic effects), polyclonal selection quality of the must RT-qPCR
Contexto Educativo
Citação
Carvalho L, Gonçalves E, Amâncio S and Martins A (2020) Selecting Aragonez Genotypes Able to Outplay Climate Change–Driven Abiotic Stress. Front. Plant Sci. 11:599230
Editora
Frontiers in Plant Science