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Dynamic regulation of Grapevine’s microRNAs in response to Mycorrhizal Symbiosis and high temperature

2023-03Journal article Open access
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dc.contributor.authorCampos, Catarina
dc.contributor.authorCoito, João Lucas
dc.contributor.authorCardoso, Hélia
dc.contributor.authorSilva, Jorge Marques da
dc.contributor.authorPereira, Helena Sofia
dc.contributor.authorViegas, Wanda
dc.contributor.authorNogales, Amaia
dc.date.accessioned2024-03-05T17:12:48Z
dc.date.available2024-03-05T17:12:48Z
dc.date.issued2023-03
dc.description.abstractMicroRNAs (miRNAs) are non-coding small RNAs that play crucial roles in plant development and stress responses and can regulate plant interactions with beneficial soil microorganisms such as arbuscular mycorrhizal fungi (AMF). To determine if root inoculation with distinct AMF species affected miRNA expression in grapevines subjected to high temperatures, RNA-seq was conducted in leaves of grapevines inoculated with either Rhizoglomus irregulare or Funneliformis mosseae and exposed to a high-temperature treatment (HTT) of 40 C for 4 h per day for one week. Our results showed that mycorrhizal inoculation resulted in a better plant physiological response to HTT. Amongst the 195 identified miRNAs, 83 were considered isomiRs, suggesting that isomiRs can be biologically functional in plants. The number of differentially expressed miRNAs between temperatures was higher in mycorrhizal (28) than in non-inoculated plants (17). Several miR396 family members, which target homeobox-leucine zipper proteins, were only upregulated by HTT in mycorrhizal plants. Predicted targets of HTT-induced miRNAs in mycorrhizal plants queried to STRING DB formed networks for Cox complex, and growth and stress-related transcription factors such as SQUAMOSA promoter-binding-like-proteins, homeobox-leucine zipper proteins and auxin receptors. A further cluster related to DNA polymerase was found in R. irregulare inoculated plants. The results presented herein provide new insights into miRNA regulation in mycorrhizal grapevines under heat stress and can be the basis for functional studies of plant-AMF-stress interactions.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCampos, C.; Coito, J.L.; Cardoso, H.; Marques da Silva, J.; Pereira, H.S.; Viegas,W.; Nogales, A. Dynamic regulation of Grapevine’s microRNAs in response to Mycorrhizal Symbiosis and high temperature. Plants 2023, 12, 982.pt_PT
dc.identifier.doi10.3390/plants12050982pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/30279
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMediterranean Institute for Agriculture, Environment and Development
dc.relationBiosystems & Integrative Sciences Institute
dc.relationLinking Landscape, Environment, Agriculture and Food
dc.relation.publisherversionhttps://www.mdpi.com/journal/plantspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectVitis vinifera L.pt_PT
dc.subjectmicroRNAspt_PT
dc.subjectRhizoglomus irregularept_PT
dc.subjectFunneliformis mosseaept_PT
dc.subjectheat-stresspt_PT
dc.subjectstress responsept_PT
dc.titleDynamic regulation of Grapevine’s microRNAs in response to Mycorrhizal Symbiosis and high temperaturept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMediterranean Institute for Agriculture, Environment and Development
oaire.awardTitleBiosystems & Integrative Sciences Institute
oaire.awardTitleLinking Landscape, Environment, Agriculture and Food
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Projetos de Investigação Científica e Desenvolvimento Tecnológico - 2014/PTDC%2FAGR-PRO%2F0676%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05183%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04046%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04129%2F2020/PT
oaire.citation.issue5pt_PT
oaire.citation.startPageArticle number 982pt_PT
oaire.citation.titlePlantspt_PT
oaire.citation.volume12pt_PT
oaire.fundingStreamProjetos de Investigação Científica e Desenvolvimento Tecnológico - 2014
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFidalgo Oliveira Coito
person.familyNameViegas
person.familyNameNogales
person.givenNameJoão Lucas
person.givenNameWanda
person.givenNameAmaia
person.identifier.ciencia-id0216-3843-AE41
person.identifier.ciencia-id2A11-692D-B451
person.identifier.ciencia-id1D11-F52B-1291
person.identifier.orcid0000-0001-9849-2821
person.identifier.orcid0000-0003-4660-1226
person.identifier.orcid0000-0001-8260-6580
person.identifier.scopus-author-id55545849600
person.identifier.scopus-author-id6603353235
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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