| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 4.58 MB | Adobe PDF |
Advisor(s)
Abstract(s)
MicroRNAs (miRNAs) are non-coding small RNAs that play crucial roles in plant development
and stress responses and can regulate plant interactions with beneficial soil microorganisms
such as arbuscular mycorrhizal fungi (AMF). To determine if root inoculation with distinct AMF
species affected miRNA expression in grapevines subjected to high temperatures, RNA-seq was
conducted in leaves of grapevines inoculated with either Rhizoglomus irregulare or Funneliformis
mosseae and exposed to a high-temperature treatment (HTT) of 40 C for 4 h per day for one week.
Our results showed that mycorrhizal inoculation resulted in a better plant physiological response
to HTT. Amongst the 195 identified miRNAs, 83 were considered isomiRs, suggesting that isomiRs
can be biologically functional in plants. The number of differentially expressed miRNAs between
temperatures was higher in mycorrhizal (28) than in non-inoculated plants (17). Several miR396
family members, which target homeobox-leucine zipper proteins, were only upregulated by HTT
in mycorrhizal plants. Predicted targets of HTT-induced miRNAs in mycorrhizal plants queried to
STRING DB formed networks for Cox complex, and growth and stress-related transcription factors
such as SQUAMOSA promoter-binding-like-proteins, homeobox-leucine zipper proteins and auxin
receptors. A further cluster related to DNA polymerase was found in R. irregulare inoculated plants.
The results presented herein provide new insights into miRNA regulation in mycorrhizal grapevines
under heat stress and can be the basis for functional studies of plant-AMF-stress interactions.
Description
Keywords
Vitis vinifera L. microRNAs Rhizoglomus irregulare Funneliformis mosseae heat-stress stress response
Pedagogical Context
Citation
Campos, C.; Coito, J.L.; Cardoso, H.; Marques da Silva, J.; Pereira, H.S.; Viegas,W.; Nogales, A. Dynamic regulation of Grapevine’s microRNAs in response to Mycorrhizal Symbiosis and high temperature. Plants 2023, 12, 982.
Publisher
MDPI