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Phosphoneurofilament heavy chain and N-glycomics from the cerebrospinal fluid in amyotrophic lateral sclerosis

2015Journal article Restricted access
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dc.contributor.authorGonçalves, Margarida
dc.contributor.authorTillack, Linda
dc.contributor.authorCarvalho, Mamede
dc.contributor.authorPinto, Susana
dc.contributor.authorConradt, Harald S.
dc.contributor.authorCosta, Júlia
dc.date.accessioned2022-03-18T17:02:08Z
dc.date.available2022-03-18T17:02:08Z
dc.date.issued2015
dc.description© 2014 Elsevier B.V. All rights reserved.pt_PT
dc.description.abstractBackground: Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disease of the motor neuron for which no clinically validated biomarkers have been identified. Methods: We have quantified by ELISA the biomarker phosphoneurofilament heavy chain (pNFH) in the cerebrospinal fluid (CSF) of ALS patients (n=29) and age-matched control patients with other diseases (n=19) by ELISA. Furthermore, we compared protein N-glycosylation of the CSF in ALS patients and controls, by applying a glycomics approach based on liquid chromatography and mass spectrometry. Results: pNFH levels were significantly higher in ALS patients in comparison with controls (P<0.0001) in particular in fast progressors. The N-glycans found in the CSF were predominantly complex diantennary with sialic acid in α2,3- and α2,6-linkage, and bisecting N-acetylglucosamine-containing structures as well as peripherally fucosylated structures were found. As compared with controls the ALS group had a significant increase of a peak composed of the monosialylated diantennary glycans A2G2S(6)1 and FA2G2S(3)1 (P=0.0348). Conclusions: Our results underscore the value of pNFH as a biomarker in ALS. In addition, we identified a variation of the N-glycosylation pattern in ALS, suggesting that this change should be explored in future studies as potential biomarker.pt_PT
dc.description.sponsorshipThis is an EU Joint Programme — Neurodegenerative Disease Research (JPND) project. The project is supported through the following funding organisations under the aegis of JPND — www.jpnd.eu: France, Agence Nationale de la Recherche; Germany, Bundesministerium für Bildung und Forschung; Ireland, Health Research Board; Italy, Ministero della Salute; The Netherlands, The Netherlands Organisation for Health Research and Development; Poland, Narodowe Centrum Badań i Rozwoju; Portugal, Fundação para a Ciência e a Tecnologia (JPND/0002/2011; JPND/0003/2011); Spain, Ministerio de Ciencia e Innovación; Switzerland, Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung; and the Turkey, Tübitak; United Kingdom, Medical Research Council. We also acknowledge funding from Fundação para a Ciência e a Tecnologia, Pest-OE/EQB/LA0004/2011, and ENMed/0001/2013, Portugal.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationClin Chim Acta. 2015 Jan 1;438:342-349pt_PT
dc.identifier.doi10.1016/j.cca.2014.09.011pt_PT
dc.identifier.eissn1873-3492
dc.identifier.issn0009-8981
dc.identifier.urihttp://hdl.handle.net/10451/51837
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationSampling and biomarker OPtimization and Harmonization In Amyotrophic Lateral Sclerosis (ALS) and other motor neuron diseases (SOPHIA)
dc.relationStrategic Project - LA 4 - 2011-2012
dc.relationExossomas optimizados para tratamento de glioma
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/clinica-chimica-actapt_PT
dc.subjectAmyotrophic lateral sclerosispt_PT
dc.subjectBiomarkerpt_PT
dc.subjectCerebrospinal fluidpt_PT
dc.subjectGlycomicspt_PT
dc.subjectGlycoproteinspt_PT
dc.subjectPhosphoneurofilament heavy chainpt_PT
dc.titlePhosphoneurofilament heavy chain and N-glycomics from the cerebrospinal fluid in amyotrophic lateral sclerosispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSampling and biomarker OPtimization and Harmonization In Amyotrophic Lateral Sclerosis (ALS) and other motor neuron diseases (SOPHIA)
oaire.awardTitleStrategic Project - LA 4 - 2011-2012
oaire.awardTitleExossomas optimizados para tratamento de glioma
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/JPND%2F0002%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/JPND%2F0003%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0004%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/ENMed%2F0001%2F2013/PT
oaire.citation.endPage349pt_PT
oaire.citation.startPage342pt_PT
oaire.citation.titleClinica Chimica Actapt_PT
oaire.citation.volume438pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
person.familyNamede Carvalho
person.familyNamePinto
person.givenNameMamede
person.givenNameSusana
person.identifier593760
person.identifier.ciencia-idD31B-E89C-CB94
person.identifier.orcid0000-0001-7556-0158
person.identifier.orcid0000-0002-0727-5897
person.identifier.ridL-8394-2019
person.identifier.scopus-author-id7101893769
person.identifier.scopus-author-id23397978500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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