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Advisor(s)
Abstract(s)
The availability of phenotyping tools to assist breeding programs in the selection of highquality
crop seeds is of obvious interest with consequences for both seed producers and
consumers. Seed germination involves the activation of several metabolic pathways,
such as cellular respiration to provide the required ATP and reducing power. This
work tested the applicability of calorespirometry, the simultaneous measurement of
heat and CO2 rates, as a phenotyping tool to assess seed respiratory properties
as a function of temperature. The effect of temperature on seed germination was
evaluated after 16 h of seed imbibition by calorespirometric experiments performed
in isothermal mode at 15, 20, 25, and 28 C on the seeds of three cultivars of peas
(Pisum sativum L.) commonly used in conventional agriculture (cvs. ‘Rondo’, ‘Torta de
Quebrar’, and ‘Maravilha d’América’). Significant differences in metabolic heat rate and
CO2 production rate (RCO2) as well as in the temperature responses of these parameters
were found among the three cultivars. A seed germination trial was conducted during
the 6 days of imbibition to evaluate the predictive power of the parameters derived
from the calorespirometric measurements. The germination trial showed that the optimal
germination temperature was 20 C and low germination rates were observed at extreme
temperatures (15 or 28 C). The cv. ‘Torta de Quebrar’ showed significantly higher
germination in comparison with the other two cultivars at all three temperatures. In
comparison with the other two cultivars, ‘Torta de Quebrar’ has the lowest metabolic
heat and CO2 rates and the smallest temperature dependence of these measured
parameters. Additionally, ‘Torta de Quebrar’ has the lowest values of growth rate and carbon use efficiency calculated from the measured variables. These data suggest
that calorespirometry is a useful tool for phenotyping physiologic efficiency at different
temperatures during early germination stages, and can determine the seeds with the
highest resilience to temperature variation, in this case ‘Torta de Quebrar’
Description
Original Research
Keywords
phenotyping alternative oxidase germination metabolism calorespirometry Fabaceae pea
Pedagogical Context
Citation
Rodrigues L, Nogales A, Hansen LD, Santos F, Rato AE and Cardoso H (2022) Exploring the Applicability of Calorespirometry to Assess Seed Metabolic Stability Upon Temperature Stress Conditions—Pisum sativum L. Used as a Case Study. Front. Plant Sci. 13:827117
Publisher
Frontiers