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Fluoxetine Arrests Growth of the Model Diatom Phaeodactylum tricornutum by Increasing Oxidative Stress and Altering Energetic and Lipid Metabolism

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dc.contributor.authorFeijão, Eduardo
dc.contributor.authorCruz de Carvalho, Ricardo
dc.contributor.authorDuarte, Irina A.
dc.contributor.authorMatos, Ana Rita
dc.contributor.authorCabrita, Maria Teresa
dc.contributor.authorNovais, Sara C.
dc.contributor.authorLemos, Marco F. L.
dc.contributor.authorCaçador, Isabel
dc.contributor.authorMarques, João Carlos
dc.contributor.authorReis-Santos, Patrick
dc.contributor.authorFonseca, Vanessa F.
dc.contributor.authorDuarte, Bernardo
dc.date.accessioned2021-05-10T13:55:35Z
dc.date.available2021-05-10T13:55:35Z
dc.date.issued2020
dc.description.abstractPharmaceutical residues impose a new and emerging threat to aquatic environments and its biota. One of the most commonly prescribed pharmaceuticals is the antidepressant fluoxetine, a selective serotonin re-uptake inhibitor that has been frequently detected, in concentrations up to 40 μg L-1, in aquatic ecosystems. The present study aims to investigate the ecotoxicity of fluoxetine at environmentally relevant concentrations (0.3, 0.6, 20, 40, and 80 μg L-1) on cell energy and lipid metabolism, as well as oxidative stress biomarkers in the model diatom Phaeodactylum tricornutum. Exposure to higher concentrations of fluoxetine negatively affected cell density and photosynthesis through a decrease in the active PSII reaction centers. Stress response mechanisms, like β-carotene (β-car) production and antioxidant enzymes [superoxide dismutase (SOD) and ascorbate peroxidase (APX)] up-regulation were triggered, likely as a positive feedback mechanism toward formation of fluoxetine-induced reactive oxygen species. Lipid peroxidation products increased greatly at the highest fluoxetine concentration whereas no variation in the relative amounts of long chain polyunsaturated fatty acids (LC-PUFAs) was observed. However, monogalactosyldiacylglycerol-characteristic fatty acids such as C16:2 and C16:3 increased, suggesting an interaction between light harvesting pigments, lipid environment, and photosynthesis stabilization. Using a canonical multivariate analysis, it was possible to evaluate the efficiency of the application of bio-optical and biochemical techniques as potential fluoxetine exposure biomarkers in P. tricornutum. An overall classification efficiency to the different levels of fluoxetine exposure of 61.1 and 88.9% were obtained for bio-optical and fatty acids profiles, respectively, with different resolution degrees highlighting these parameters as potential efficient biomarkers. Additionally, the negative impact of this pharmaceutical molecule on the primary productivity is also evident alongside with an increase in respiratory oxygen consumption. From the ecological point of view, reduction in diatom biomass due to continued exposure to fluoxetine may severely impact estuarine and coastal trophic webs, by both a reduction in oxygen primary productivity and reduced availability of key fatty acids to the dependent heterotrophic upper levels.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFeijão, E., Cruz de Carvalho, R., Duarte. I. A., Matos, A.R., Cabrita, M. T., Novais, S. C., Lemos, M. F. L., Caçador, I., Marques, J. C., Reis-Santos, P., Fonseca, V. F. & Duarte, B. (2020). Fluoxetine Arrests Growth of the Model Diatom Phaeodactylum tricornutum by Increasing Oxidative Stress and Altering Energetic and Lipid Metabolism. Frontiers in Microbiology, 11, 1803. https://doi.org/10.3389/fmicb.2020.01803pt_PT
dc.identifier.doi10.3389/fmicb.2020.01803pt_PT
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10451/47720
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Mediapt_PT
dc.relationCTA-AMB/30056/2017pt_PT
dc.relationMarine and Environmental Sciences Centre
dc.relationBiosystems & Integrative Sciences Institute
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fmicb.2020.01803/fullpt_PT
dc.subjectPharmaceuticalspt_PT
dc.subjectAntidepressantpt_PT
dc.subjectMicroalgaept_PT
dc.subjectEcotoxicitypt_PT
dc.subjectPhotobiologypt_PT
dc.subjectCell energypt_PT
dc.subjectBiomarkerspt_PT
dc.subjectFatty acid profilept_PT
dc.titleFluoxetine Arrests Growth of the Model Diatom Phaeodactylum tricornutum by Increasing Oxidative Stress and Altering Energetic and Lipid Metabolismpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPTDC/MAR-EST/3048/2014
oaire.awardNumberUIDB/04292/2020
oaire.awardNumberUID/Multi/04046/2013
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleBiosystems & Integrative Sciences Institute
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR-EST%2F3048%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04046%2F2013/PT
oaire.citation.startPage1803pt_PT
oaire.citation.titleFrontiers in Microbiologypt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCabrita
person.givenNameMaria Teresa
person.identifier349469
person.identifier.ciencia-id3E17-0125-F909
person.identifier.orcid0000-0002-4323-4945
person.identifier.ridB-5746-2014
person.identifier.scopus-author-id6602224932
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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