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Fighting S. aureus catheter-related infections with sophorolipids: Electing an antiadhesive strategy or a release one?

2021-12Journal article Restricted access
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dc.contributor.authorMendes, Rita M.
dc.contributor.authorFrancisco, Ana Paula
dc.contributor.authorCarvalho, Filomena A.
dc.contributor.authorDardouri, Maïssa
dc.contributor.authorCosta, Bruna
dc.contributor.authorBettencourt, Ana
dc.contributor.authorCosta, Judite
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorCosta, Fabíola
dc.contributor.authorRibeiro, Isabel A. C.
dc.date.accessioned2023-09-13T11:02:16Z
dc.date.available2023-09-13T11:02:16Z
dc.date.issued2021-12
dc.date.updated2023-02-27T15:34:27Z
dc.description.abstractStaphylococcus aureus medical devices related-infections, such as blood stream catheter are of major concern. Their prevention is compulsory and strategies, not prone to the development of resistance, to prevent S. aureus biofilms on catheter surfaces (e.g. silicone) are needed. In this work two different approaches using sophorolipids were studied to prevent S. aureus biofilm formation on medical grade silicone: i) an antiadhesive strategy through covalent bond of sophorolipids to the surface; ii) and a release strategy using isolated most active sophorolipids. Sophorolipids produced by Starmerella bombicola, were characterized by UHPLC-MS and RMN, purified by automatic flash chromatography and tested for their antimicrobial activity towards S. aureus. Highest antimicrobial activity was observed for C18:0 and C18:1 diacetylated lactonic sophorolipids showing a MIC of 50 μg mL−1. Surface modification with acidic or lactonic sophorolipids when evaluating the anti-adhesive or release strategy, respectively, was confirmed by contact angle, FTIR-ATR and AFM analysis. When using a mixture of acidic sophorolipids covalently bonded to silicone surface as antiadhesive strategy cytocompatible surfaces were obtained and a reduction of 90 % on biofilm formation was observed. Nevertheless, if a release strategy is adopted with purified lactonic sophorolipids a higher effect is achieved. Most promising compound was C18:1 diacateylated lactonic sophorolipid that showed no cellular viability reduction when a concentration of 1.5 mg mL−1 was selected and a reduction on biofilm around 5 log units. Results reinforce the applicability of these antimicrobial biosurfactants on preventing biofilms and disclose that their antimicrobial effect is imperative when comparing to their antiadhesive properties.pt_PT
dc.description.sponsorshipThe authors thank Fundação para a Ciência e Tecnologia (FCT) for the financial support under Projects PTDC/BTM-SAL/29335/2017, UIDB/04138/2020, UIDP/04138/2020 (iMed.ULisboa) and Portugal 2020 for the Portuguese Mass Spectrometry Network (Rede Nacional de Espectrometria de Massa RNEM; LISBOA 01 0145 FEDER 402 022125). The authors also acknowledge the Designer Nuno Monge for the artwork, Isabel Dias Nogueira (Microlab, Instituto Superior Técnico, Lisbon University) for the technical assistance with the SEM analysis, Joana Carola for the assistance with the UHPLC-MS equipment (iMed.Ulisboa) and Professor Rui Moreira (FFULisboa) for allowing the use of the automatic flash chromatography equipment. Moreover, this publication is partially based upon networking originated from COST Action "European Network of Multidisciplinary Research to Improve the Urinary Stents- CA16217", supported by COST (European Cooperation in Science and Technology). In memory of our Dear colleague and friend Judite.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMendes RM, Francisco AP, Carvalho FA, Dardouri M, Costa B, Bettencourt AF, et al. Fighting S. aureus catheter-related infections with sophorolipids: Electing an antiadhesive strategy or a release one? Colloids and Surfaces B: Biointerfaces [Internet]. 1 de dezembro de 2021;208:112057. Disponível em: https://www.sciencedirect.com/science/article/pii/S0927776521005014pt_PT
dc.identifier.doi10.1016/j.colsurfb.2021.112057pt_PT
dc.identifier.issn0927-7765
dc.identifier.slugcv-prod-2779240
dc.identifier.urihttp://hdl.handle.net/10451/59252
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationUIDB/04138/2020pt_PT
dc.relationAntiBiofilm features of Glycolipid-functionalized Silicone Catheters towards Healthcare-associated infections prevention
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0927776521005014pt_PT
dc.subjectS. aureuspt_PT
dc.subjectSelf-assembling monolayerspt_PT
dc.subjectAntibacterial surfacept_PT
dc.subjectBiofilmpt_PT
dc.subjectAnti-adhesivept_PT
dc.subjectReleasept_PT
dc.titleFighting S. aureus catheter-related infections with sophorolipids: Electing an antiadhesive strategy or a release one?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAntiBiofilm features of Glycolipid-functionalized Silicone Catheters towards Healthcare-associated infections prevention
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTM-SAL%2F29335%2F2017/PT
oaire.citation.startPage112057pt_PT
oaire.citation.titleColloids and Surfaces B: Biointerfacespt_PT
oaire.citation.volume208pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameFrancisco
person.familyNameDARDOURI
person.familyNameVieira Costa
person.familyNameCampos Simão Bettencourt
person.familyNameCosta
person.familyNameDiogo Gonçalves
person.familyNameTavares Alves da Costa Moutinho
person.familyNameRibeiro
person.givenNameAna Paula
person.givenNameMaïssa
person.givenNameBruna Filipa
person.givenNameAna Francisca
person.givenNameJudite
person.givenNameLídia Maria
person.givenNameFabíola Maria
person.givenNameIsabel
person.identifierM-4178-2013
person.identifierM-4219-2013
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person.identifier.ciencia-idAC1C-68E7-6E81
person.identifier.ciencia-idE31A-26B8-4AC6
person.identifier.orcid0000-0001-6602-2075
person.identifier.orcid0000-0002-0797-8509
person.identifier.orcid0000-0002-2409-5847
person.identifier.orcid0000-0002-8498-5892
person.identifier.orcid0000-0001-8557-4799
person.identifier.orcid0000-0002-6799-2740
person.identifier.orcid0000-0001-8981-7049
person.identifier.orcid0000-0002-7110-6393
person.identifier.ridA-8359-2014
person.identifier.ridA-6098-2014
person.identifier.scopus-author-id57237689900
person.identifier.scopus-author-id6603687672
person.identifier.scopus-author-id7402461239
person.identifier.scopus-author-id22836446000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid7211-22BA-86AD | Lídia Maria Diogo Gonçalves
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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