Publicação
Oxidative injury in V79 Chinese hamster cells: protective
| dc.contributor.author | Fernandes, Ana S. | |
| dc.contributor.author | Serejo, João | |
| dc.contributor.author | Gaspar, Jorge | |
| dc.contributor.author | Cabral, Fátima | |
| dc.contributor.author | Bettencourt, Ana F. | |
| dc.contributor.author | Rueff, José | |
| dc.contributor.author | Castro, Matilde | |
| dc.contributor.author | Costa, Judite | |
| dc.contributor.author | Oliveira, Nuno G. | |
| dc.date.accessioned | 2013-06-03T14:54:07Z | |
| dc.date.available | 2013-06-03T14:54:07Z | |
| dc.date.issued | 2010 | |
| dc.description.abstract | Oxidative cell injury could be induced by different reactive oxygen species (ROS) operating in multiple pathways. The present work is focused on three different models of oxidative stress: the xanthine/xanthine oxidase system (XXO), an extracellular superoxide anion generator; tert-butylhydroperoxide (TBHP), an analogue of lipid hydroperoxides; and doxorubicin (Dox), an anticancer drug. Superoxide and peroxyl radicals, among other ROS, could be effectively scavenged by MnTM-4-PyP, a polyfunctional catalytic antioxidant. In this report, we have addressed the role of MnTM-4-PyP on the protection against the cytotoxicity induced by the three aforementioned oxidants. The effect of MnTM-4-PyP (0.1–100 μM) was evaluated in V79 fibroblasts using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide reduction and the crystal violet assays, as well as the mitotic index. Also, the generation of intracellular ROS was studied by the fluorescent probe dihydroethidium. MnTM-4-PyP has shown significant protective effects against the cytotoxicity of XXO and TBHP, increasing the cell viability in approximately 40% and reducing the intracellular level of ROS. However, no considerable protection occurred against Dox. The three oxidants caused a mitotic index reduction that was not altered by MnTM-4-PyP. In summary, MnTM-4-PyP appears to be a promising agent for the protection against oxidative injury. However, it has shown differential responses, reinforcing the need to study different experimental models for the adequate evaluation of its potentialities as a catalytic antioxidant. | por |
| dc.description.sponsorship | A.S.F. acknowledges Fundação para a Ciência e a Tecnologia, Portugal for the financial support (PhD grant SFRH/BD/28773/2006) | por |
| dc.identifier.citation | Cell Biology and Toxicology (2010) 26:91–101 | por |
| dc.identifier.issn | 0742-2091 | |
| dc.identifier.issn | 1573-6822 | |
| dc.identifier.uri | http://dx.doi.org/10.1007/s10565-009-9120-3 | |
| dc.identifier.uri | http://hdl.handle.net/10451/8609 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Springer | por |
| dc.relation.publisherversion | http://link.springer.com/article/10.1007%2Fs10565-009-9120-3 | por |
| dc.subject | Cytotoxicity | por |
| dc.subject | Doxorubicin | por |
| dc.subject | MnTM-4-PyP | por |
| dc.subject | Superoxide dismutase mimetic | por |
| dc.subject | Tert-butylhydroperoxide | por |
| dc.subject | Xanthine-xanthine oxidase | por |
| dc.title | Oxidative injury in V79 Chinese hamster cells: protective | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 101 | por |
| oaire.citation.startPage | 91 | por |
| oaire.citation.title | Cell Biology and Toxicology | por |
| oaire.citation.volume | 26 | por |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |