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Gymnodinium catenatum Graham isolated from the Portuguese coast: Toxin content and genetic characterization

2015Journal article Restricted access
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dc.contributor.authorSilva, Teresa
dc.contributor.authorCaeiro, Maria F.
dc.contributor.authorCosta, Pedro Reis
dc.contributor.authorAmorim, Ana
dc.date.accessioned2019-06-25T15:44:04Z
dc.date.available2019-06-25T15:44:04Z
dc.date.issued2015
dc.description.abstractThe bloom forming marine dinoflagellate Gymnodinium catenatum Graham has been linked to paralytic shellfish poisoning (PSP) outbreaks in humans. Along the Portuguese coast (NE Atlantic), G. catenatum shows a complex bloom pattern, raising questions about the origin and affinities of each bloom population. In this work, the variability within six cultured strains of G. catenatum isolated from Portuguese coastal waters (S coast, W coast and NW coast), between 1999 and 2011, was investigated. The strains were analyzed for toxin profiling and intra-specific genetic diversity. Regarding the toxin profile, differences recorded between strains could not be assigned to the time of isolation or geographical origin. The parameter that most influenced the toxin profile was the life-cycle stage that originated the culture: vegetative cell versus hypnozygote (resting cyst). At the genetic level, all strains showed similar sequences for the D1-D2 region of the large subunit (LSU) of the nuclear ribosomal DNA (rDNA) and shared complete identity with strains from Spain, Algeria, China and Australia. Conversely, we did not find a total identity match for the ITS-5.8S nuclear rDNA fragment. After sequence analysis, two guanine/adenine (R) single nucleotide polymorphisms (SNP 1 and 2) were detected for all strains, in the ITS1 region. This species has been reported to present very conservative LSU and ITS-5.8S rDNA regions, though with few SNP, including SNP1 of this study, already attributed to strains from certain locations. The SNP here described characterize G. catenatum populations from Portuguese waters and may represent valuable genetic markers for studies on the phylogeography of this species.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.hal.2015.07.008pt_PT
dc.identifier.issn1568-9883
dc.identifier.urihttp://hdl.handle.net/10451/38874
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectGymnodinium catenatumpt_PT
dc.subjectIntra-specific variabilitypt_PT
dc.subjectPST profilept_PT
dc.subjectStrain originpt_PT
dc.subjectSingle nucleotide polymorphismpt_PT
dc.subjectPortugalpt_PT
dc.titleGymnodinium catenatum Graham isolated from the Portuguese coast: Toxin content and genetic characterizationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage104pt_PT
oaire.citation.startPage94pt_PT
oaire.citation.titleHarmful Algaept_PT
oaire.citation.volume48pt_PT
person.familyNameSilva
person.familyNameAmorim
person.givenNameTeresa
person.givenNameAna
person.identifier.ciencia-idBB11-EA34-DE3E
person.identifier.orcid0000-0003-4785-5383
person.identifier.orcid0000-0002-9612-4280
person.identifier.ridAAA-2615-2020
person.identifier.scopus-author-id7006426815
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication10289fb1-941d-45ba-a3d7-c4e00b91c85a
relation.isAuthorOfPublication5e0bed63-54bf-43c6-8275-5d38ea6c1c2c
relation.isAuthorOfPublication.latestForDiscovery10289fb1-941d-45ba-a3d7-c4e00b91c85a

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