Logo do repositório
 
Publicação

Genetic screen in Drosophila melanogaster uncovers a novel set of genes required for embryonic epithelial repair

2010Artigo científico Acesso restrito
Ver registo simples
dc.contributor.authorCampos, Isabel
dc.contributor.authorGeiger, Jennifer
dc.contributor.authorSantos, Ana Catarina
dc.contributor.authorCarlos, Vanessa
dc.contributor.authorJacinto, Antonio
dc.date.accessioned2021-05-31T14:54:15Z
dc.date.available2021-05-31T14:54:15Z
dc.date.issued2010
dc.descriptionCopyright © 2010 by the Genetics Society of America.pt_PT
dc.description.abstractThe wound healing response is an essential mechanism to maintain the integrity of epithelia and protect all organisms from the surrounding milieu. In the "purse-string" mechanism of wound closure, an injured epithelial sheet cinches its hole closed via an intercellular contractile actomyosin cable. This process is conserved across species and utilized by both embryonic as well as adult tissues, but remains poorly understood at the cellular level. In an effort to identify new players involved in purse-string wound closure we developed a wounding strategy suitable for screening large numbers of Drosophila embryos. Using this methodology, we observe wound healing defects in Jun-related antigen (encoding DJUN) and scab (encoding Drosophila alphaPS3 integrin) mutants and performed a forward genetics screen on the basis of insertional mutagenesis by transposons that led to the identification of 30 lethal insertional mutants with defects in embryonic epithelia repair. One of the mutants identified is an insertion in the karst locus, which encodes Drosophila beta(Heavy)-spectrin. We show beta(Heavy)-spectrin (beta(H)) localization to the wound edges where it presumably exerts an essential function to bring the wound to normal closure.pt_PT
dc.description.sponsorshipThis work was supported by Fundação para a Ciência e a Tecnologia (I.C. SFRH/BPD/17729/2004; J.A.G. SFRH/BD/21895/2005, PTDC/BIA-BCM/65872/2006, A.C.S. SFRH/BPD/24976/2005), European Union Framework Programs (LSHM-CT-2003-504468, ERC-2007-StG), and Human Frontiers Science Program (RGP21/2007)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGenetics. 2010 Jan;184(1):129-140pt_PT
dc.identifier.doi10.1534/genetics.109.110288pt_PT
dc.identifier.eissn1943-2631
dc.identifier.issn0016-6731
dc.identifier.urihttp://hdl.handle.net/10451/48265
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Presspt_PT
dc.relationLSHM-CT-2003-504468pt_PT
dc.relationRC-2007-StGpt_PT
dc.relationRGP21/2007pt_PT
dc.relationIDENTIFICATION AND CHARACTERIZATION OF NEW GENES REQUIRED FOR DORSAL CLOSURE AND WOUND HEALING IN D.MELANOGASTER
dc.relation.publisherversionhttps://academic.oup.com/geneticspt_PT
dc.titleGenetic screen in Drosophila melanogaster uncovers a novel set of genes required for embryonic epithelial repairpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberSFRH/BPD/17729/2004
oaire.awardNumberSFRH/BD/21895/2005
oaire.awardNumberPTDC/BIA-BCM/65872/2006
oaire.awardNumberSFRH/BPD/24976/2005
oaire.awardTitleIDENTIFICATION AND CHARACTERIZATION OF NEW GENES REQUIRED FOR DORSAL CLOSURE AND WOUND HEALING IN D.MELANOGASTER
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F17729%2F2004/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F21895%2F2005/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BCM%2F65872%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F24976%2F2005/PT
oaire.citation.endPage140pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage129pt_PT
oaire.citation.titleGeneticspt_PT
oaire.citation.volume184pt_PT
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
person.familyNameCampos
person.givenNameIsabel
person.identifier.ciencia-id1916-7A21-7F30
person.identifier.orcid0000-0002-1541-8552
person.identifier.scopus-author-id55217099800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationbe2c828c-a9f9-4cc9-8afb-d937d2c3112e
relation.isAuthorOfPublication.latestForDiscoverybe2c828c-a9f9-4cc9-8afb-d937d2c3112e
relation.isProjectOfPublicationad7372cb-e2d2-48ee-8a95-81cdb7e26ec1
relation.isProjectOfPublication7735b59a-3fd4-4640-80e0-34fabe0aea94
relation.isProjectOfPublication5e1d0252-3809-4777-8b1f-e2571c75034e
relation.isProjectOfPublication0fa579f8-a0e2-4c72-8478-f254d381843c
relation.isProjectOfPublication.latestForDiscovery7735b59a-3fd4-4640-80e0-34fabe0aea94

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
Drosophila_melanogaster.pdf
Tamanho:
4.43 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

IMM - Artigos em Revistas Internacionais
FM - Artigos em Revistas Internacionais