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Diabetic foot infections : application of a nisin-biogel to complement the activity of conventional antibiotics and antiseptics against Staphylococcus aureus biofilms

2019-07-24Journal article Open access
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dc.contributor.authorSantos, Raquel
dc.contributor.authorRuza, Diana
dc.contributor.authorCunha, Eva
dc.contributor.authorTavares, Luis
dc.contributor.authorOliveira, Manuela
dc.date.accessioned2021-10-28T14:56:35Z
dc.date.available2021-10-28T14:56:35Z
dc.date.issued2019-07-24
dc.descriptionResearch Areas: Science & Technology - Other Topicspt_PT
dc.description.abstractABSTRACT - Background Diabetic foot infections (DFIs) are a frequent complication of Diabetes mellitus and a major cause of nontraumatic limb amputations. The Gram-positive bacterium Staphylococcus aureus, known for its resilient biofilms and antibiotic resistant profile, is the most frequent DFI pathogen. It is urgent to develop innovative treatments for these infections, being the antimicrobial peptide (AMP) nisin a potential candidate. We have previously proposed the use of a guar gum biogel as a delivery system for nisin. Here, we evaluated the potential of the nisin-biogel to enhance the efficacy of conventional antibiotics and antiseptics against DFIs S. aureus clinical isolates. Methods A collection of 23 S. aureus strains isolated from DFI patients, including multidrug- and methicillin-resistant strains, was used. The antimicrobial activity of the nisin-biogel was tested alone and in different combinations with the antiseptic chlorhexidine and the antibiotics clindamycin, gentamicin and vancomycin. Isolates' in vitro susceptibility to the different protocols was assessed using broth microdilution methods in order to determine their ability to inhibit and/or eradicate established S. aureus biofilms. Antimicrobials were added to the 96-well plates every 8 h to simulate a typical DFI treatment protocol. Statistical analysis was conducted using RCBD ANOVA in SPSS. Results The nisin-biogel showed a high antibacterial activity against biofilms formed by DFI S. aureus. The combined protocol using nisin-biogel and chlorhexidine presented the highest efficacy in biofilm formation inhibition, significantly higher (p<0.05) than the ones presented by the antibiotics-based protocols tested. Regarding biofilm eradication, there were no significant differences (p>0.05) between the activity of the combination nisin-biogel plus chlorhexidine and the conventional antibiotic-based protocols. Conclusions Results provide a valuable contribution for the development of complementary strategies to conventional antibiotics protocols. A combined protocol including chlorhexidine and nisinbiogel could be potentially applied in medical centres, contributing for the reduction of antibiotic administration, selection pressure on DFI pathogens and resistance strains dissemination.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSantos R, Ruza D, Cunha E, Tavares L, Oliveira M. 2019. Diabetic foot infections: application of a nisin-biogel to complement the activity of conventional antibiotics and antiseptics against Staphylococcus aureus biofilms. PLoS ONE 14(7):e0220000pt_PT
dc.identifier.doi10.1371/journal. pone.0220000pt_PT
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.5/22439
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMeghan Scobee Blackledge, High Point University, UNITED STATESpt_PT
dc.relationUID/CVT/276/2019 (CIISA - Centro de Investigacao Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa)pt_PT
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0220000pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHost-defense peptidespt_PT
dc.subjectin-vitro activitiespt_PT
dc.subjectChlorhexidinept_PT
dc.subjectResistantept_PT
dc.subjectInterococcuspt_PT
dc.subjectVancomycinpt_PT
dc.subjectMechanismspt_PT
dc.subjectDiagnosispt_PT
dc.titleDiabetic foot infections : application of a nisin-biogel to complement the activity of conventional antibiotics and antiseptics against Staphylococcus aureus biofilmspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/149693/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/40465/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/68219/PT
oaire.citation.conferencePlaceSAN FRANCISCO, USApt_PT
oaire.citation.issuee0220000pt_PT
oaire.citation.titlePLoS ONEpt_PT
oaire.citation.volume14(7)pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamOE
oaire.fundingStreamOE
person.familyNameGonçalves da Cunha
person.familyNameMorgado Tavares
person.familyNameOliveira
person.givenNameEva Sofia
person.givenNameLuis Manuel
person.givenNameManuela
person.identifier.ciencia-id9811-7B6B-E2B9
person.identifier.ciencia-id9011-18C6-482D
person.identifier.ciencia-idBE1E-2B3B-92D2
person.identifier.orcid0000-0002-5641-4400
person.identifier.orcid0000-0001-8671-6285
person.identifier.orcid0000-0002-3910-1525
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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