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Caracterização de Enterococcus spp. isolados da boca e do coração de cães com doença periodontal
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Resumo(s)
O presente estudo pretende verificar a possível associação entre a doença periodontal e a doença cardiovascular, avaliando a presença e diversidade de Enterococcus spp. na gengiva e no coração de cães com doença periodontal. Através de métodos fenotípicos e moleculares identificaram-se 117 isolados como pertencentes ao género Enterococcus e avaliou-se a sua diversidade pela técnica de PCR-fingerprinting. Selecionaram-se 46 isolados representantes, 39 identificados como E. faecalis, 7 como E. faecium e 2 permaneceram por identificar. Para estimar o potencial de patogenicidade avaliaram-se os isolados quanto à suscetibilidade a antimicrobianos e à presença de fatores de virulência. Todos os isolados mostraram resistência à clindamicina; para a tetraciclina e a gentamicina as percentagens foram acima dos 50% e para os restantes antimicrobianos mantiveram-se abaixo desse valor. Na pesquisa de fatores de virulência 43% dos isolados revelaram-se β-hemolíticos e 23% gelatinase positivos. Para os genes de virulência pesquisados detetaram-se percentagens acima dos 50% para gelE, efaAfs, ebpA, ebpB, ebpC e gls24 e abaixo desse valor para agg, esp, efaAfm, cylA, acm e ace.
Não se verificou associação entre a doença periodontal e a endocardite bacteriana, mas foi possível verificar a presença de bactérias de importância clínica disseminadas pela boca e coração de cães em níveis relativamente elevados, sendo de suma importância prosseguir estudos no sentido de melhor compreender esta possível associação.
ABSTRACT - Characterization of Enterococcus spp. isolated from the mouth and heart of dogs with periodontal disease - The present study investigated the possible association between periodontal and cardiovascular disease, evaluating the presence and diversity of Enterococcus spp. in the gum and heart of dogs with periodontal disease. Phenotypic and molecular methods were used, yielding a total of 117 isolates identified as Enterococcus spp., evaluated for diversity by PCR-fingerprinting. 46 representative isolates were selected, 39 of which identified as E. faecalis, 7 as E. faecium and two remained unidentified. To estimate the potential pathogenicity of the isolates, they were evaluated for susceptibility to antimicrobial agents and the presence of virulence factors. All isolates showed resistance to clindamycin; for tetracycline and gentamicin percentages were above 50% and for the remaining antimicrobials remained below this value. In search of virulence factors 43% of the isolates proved to be β-hemolytic and 23% gelatinase positive. For the virulence genes surveyed, percentages observed were above 50% for gelE, efaAfs, ebpA, ebpB, ebpC and gls24 and below this value for agg, esp, efaAfm, cylA, acm and ace. It was not possible to establish an association between periodontal disease and bacterial endocarditis, but it was possible to verify the presence of bacteria of clinical importance disseminated through the mouth and heart of dogs at relatively high levels. It is extremely important to continue studies to better understand this possible association.
ABSTRACT - Characterization of Enterococcus spp. isolated from the mouth and heart of dogs with periodontal disease - The present study investigated the possible association between periodontal and cardiovascular disease, evaluating the presence and diversity of Enterococcus spp. in the gum and heart of dogs with periodontal disease. Phenotypic and molecular methods were used, yielding a total of 117 isolates identified as Enterococcus spp., evaluated for diversity by PCR-fingerprinting. 46 representative isolates were selected, 39 of which identified as E. faecalis, 7 as E. faecium and two remained unidentified. To estimate the potential pathogenicity of the isolates, they were evaluated for susceptibility to antimicrobial agents and the presence of virulence factors. All isolates showed resistance to clindamycin; for tetracycline and gentamicin percentages were above 50% and for the remaining antimicrobials remained below this value. In search of virulence factors 43% of the isolates proved to be β-hemolytic and 23% gelatinase positive. For the virulence genes surveyed, percentages observed were above 50% for gelE, efaAfs, ebpA, ebpB, ebpC and gls24 and below this value for agg, esp, efaAfm, cylA, acm and ace. It was not possible to establish an association between periodontal disease and bacterial endocarditis, but it was possible to verify the presence of bacteria of clinical importance disseminated through the mouth and heart of dogs at relatively high levels. It is extremely important to continue studies to better understand this possible association.
Descrição
Dissertação de Mestrado Integrado em Medicina Veterinária
Palavras-chave
Doença periodontal Endocardite bacteriana Enterococcus spp. Canídeos Periodontal disease Bacterial endocarditis Canids
Contexto Educativo
Citação
Tavares, M.M.P. (2014). Caracterização de Enterococcus spp. isolados da boca e do coração de cães com doença periodontal. Dissertação de Mestrado. Universidade de Lisboa, Faculdade de Medicina Veterinária, Lisboa.
Editora
Universidade de Lisboa. Faculdade de Medicina Veterinária