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Influence of dietary Spirulin inclusion and lysozyme supplementation on the longissimus lumborum muscle proteome of newly weaned piglets

2021-07-30Artigo científico Acesso aberto
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dc.contributor.authorRibeiro, D. M.
dc.contributor.authorMartins, Cátia Falcão
dc.contributor.authorKules, Josipa
dc.contributor.authorHorvatic, Anita
dc.contributor.authorGuillemin, Nicolas
dc.contributor.authorFreire, João
dc.contributor.authorEckersall, P. David
dc.contributor.authorAlmeida, André
dc.contributor.authorPrates, José A.M
dc.date.accessioned2021-11-04T17:22:17Z
dc.date.available2021-11-04T17:22:17Z
dc.date.issued2021-07-30
dc.descriptionResearch Areas: Biochemistry & Molecular Biologypt_PT
dc.description.abstractArthrospira platensis (Spirulina) is a microalga with a high content of crude protein. It has a recalcitrant cell wall that limits the accessibility of the animal endogenous enzymes to its intracellular nutrients. Enzymatic supplementation aiming to degrade cell walls could benefit microalgae digestibility. The objective of this study was to evaluate the impact of dietary Spirulina and lysozyme supplementation over the muscle proteome of piglets during the post-weaning stage. Thirty piglets were randomly distributed among three diets: control (no microalga), SP (10% Spirulina) and SP + L (10% Spirulina +0.01% lysozyme). After 4 weeks, they were sacrificed and samples of the longissimus lumborum muscle were taken. The muscle proteome was analysed using a Tandem Mass Tag (TMT)-based quantitative approach. A total of 832 proteins were identified. Three comparisons were computed: SP vs Ctrl, SP + L vs Ctrl and SP + L vs SP. They had ten, four and twelve differentially abundant proteins. Glycogen metabolism and nutrient reserves utilization are increased in the SP piglets. Structural muscle protein synthesis increased, causing higher energy requirements in SP + L piglets. Our results demonstrate the usefulness of proteomics to disclose the effect of dietary microalgae, whilst unveiling putative mechanisms derived from lysozyme supplementation. Data available via ProteomeXchange with identifier PXD024083. Significance: Spirulina, a microalga, is an alternative to conventional crops which could enhance the environmental sustainability of animal production. Due to its recalcitrant cell wall, its use requires additional measures to prevent anti-nutritional effects on the feeding of piglets in the post-weaning period, during which they endure post-weaning stress. One of such measures could be CAZyme supplementation to help degrade the cell wall during digestion. Muscle proteomics provides insightful data on the effect of dietary microalgae and enzyme activity on piglet metabolism.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRibeiro DM, Martins CF, Kules J, Horvatic A, Guillemin N, Freire JPB, Eckersall PD, Almeida AM, Prates JAM. 2021. Influence of dietary Spirulin inclusion and lysozyme supplementation on the longissimus lumborum muscle proteome of newly weaned piglets. Journal of Proteomics, 244: 104274. DOI:10.1016/j.jprot.2021.104274pt_PT
dc.identifier.doi10.1016/j.jprot.2021.104274pt_PT
dc.identifier.eissn1876-7737
dc.identifier.issn1874-3919
dc.identifier.urihttp://hdl.handle.net/10400.5/22503
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherELSEVIERpt_PT
dc.relationPTDC/CVTNUT/5931/2014pt_PT
dc.relation08/SI/3399/2015pt_PT
dc.relationUpgrading the research performance in molecular medicine at the Faculty of Veterinary Medicine University of Zagreb
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1874391921001731?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSpirulinapt_PT
dc.subjectCarbohydrate-active enzymes (CAZyme)pt_PT
dc.subjectWeaned pigletpt_PT
dc.subjectMuscle proteomept_PT
dc.titleInfluence of dietary Spirulin inclusion and lysozyme supplementation on the longissimus lumborum muscle proteome of newly weaned pigletspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleUpgrading the research performance in molecular medicine at the Faculty of Veterinary Medicine University of Zagreb
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157379/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157519/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/74495/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/621394/EU
oaire.citation.conferencePlaceNETHERLANDSpt_PT
oaire.citation.issue104274pt_PT
oaire.citation.titleJournal of Proteomicspt_PT
oaire.citation.volume244pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
oaire.fundingStreamFP7
person.familyNameRibeiro Viera Santos
person.familyNameFalcão Martins
person.familyNameBengala Freire
person.familyNameMartinho de Almeida
person.familyNameMestre Prates
person.givenNameDavid Miguel
person.givenNameCátia
person.givenNameJoão Pedro
person.givenNameAndré
person.givenNameJosé António
person.identifier.ciencia-id2714-8393-F2A3
person.identifier.ciencia-id5A14-15B0-2682
person.identifier.ciencia-id871A-F6C8-60FE
person.identifier.ciencia-id1E14-92CC-B163
person.identifier.ciencia-id2617-53FF-04CD
person.identifier.orcid0000-0002-0990-7739
person.identifier.orcid0000-0002-1017-3269
person.identifier.orcid0000-0002-1811-4378
person.identifier.orcid0000-0002-7810-3988
person.identifier.orcid0000-0003-1032-5987
person.identifier.ridK-9934-2013
person.identifier.scopus-author-id56003169500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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