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What makes umbilical cord tissue-derived mesenchymal stromal cells superior immunomodulators when compared to bone marrow derived mesenchymal stromal cells?

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dc.contributor.authorBárcia, R. N.
dc.contributor.authorSantos, J. M.
dc.contributor.authorFilipe, M.
dc.contributor.authorTeixeira, M.
dc.contributor.authorMartins, J. P.
dc.contributor.authorAlmeida, J.
dc.contributor.authorÁgua-Doce, A.
dc.contributor.authorAlmeida, S. C. P.
dc.contributor.authorVarela, A.
dc.contributor.authorPohl, S.
dc.contributor.authorDittmar, K. E. J.
dc.contributor.authorCalado, S.
dc.contributor.authorSimões, S. I.
dc.contributor.authorGaspar, M. M.
dc.contributor.authorCruz, M. E. M.
dc.contributor.authorLindenmaier, W.
dc.contributor.authorGraça, L.
dc.contributor.authorCruz, H.
dc.contributor.authorCruz, P. E.
dc.date.accessioned2022-03-16T17:06:57Z
dc.date.available2022-03-16T17:06:57Z
dc.date.issued2015
dc.descriptionCopyright © 2015 R. N. Bárcia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.pt_PT
dc.description.abstractMSCs derived from the umbilical cord tissue, termed UCX, were investigated for their immunomodulatory properties and compared to bone marrow-derived MSCs (BM-MSCs), the gold-standard in immunotherapy. Immunogenicity and immunosuppression were assessed by mixed lymphocyte reactions, suppression of lymphocyte proliferation and induction of regulatory T cells. Results showed that UCX were less immunogenic and showed higher immunosuppression activity than BM-MSCs. Further, UCX did not need prior activation or priming to exert their immunomodulatory effects. This was further corroborated in vivo in a model of acute inflammation. To elucidate the potency differences observed between UCX and BM-MSCs, gene expression related to immune modulation was analysed in both cell types. Several gene expression profile differences were found between UCX and BM-MSCs, namely decreased expression of HLA-DRA, HO-1, IGFBP1, 4 and 6, ILR1, IL6R and PTGES and increased expression of CD200, CD273, CD274, IL1B, IL-8, LIF and TGFB2. The latter were confirmed at the protein expression level. Overall, these results show that UCX seem to be naturally more potent immunosuppressors and less immunogenic than BM-MSCs. We propose that these differences may be due to increased levels of immunomodulatory surface proteins such as CD200, CD273, CD274 and cytokines such as IL1β, IL-8, LIF and TGFβ2.pt_PT
dc.description.sponsorshipThis study was partially supported by PORLisboa-FEDER (QREN 30196-ClinUCX; QREN 024765-OSCAR).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationStem Cells Int. 2015;2015:583984pt_PT
dc.identifier.doi10.1155/2015/583984pt_PT
dc.identifier.eissn1687-9678
dc.identifier.issn1687-966X
dc.identifier.urihttp://hdl.handle.net/10451/51781
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherHindawipt_PT
dc.relationQREN 30196-ClinUCXpt_PT
dc.relationQREN 024765-OSCARpt_PT
dc.relation.publisherversionhttps://www.hindawi.com/journals/sci/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleWhat makes umbilical cord tissue-derived mesenchymal stromal cells superior immunomodulators when compared to bone marrow derived mesenchymal stromal cells?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage14pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleStem Cells Internationalpt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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