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A chromosome-level genome resource for studying virulence mechanisms and evolution of the coffee rust pathogen Hemileia vastatrix
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Orientador(es)
Resumo(s)
Recurrent epidemics of coffee leaf rust, caused by the fungal pathogen Hemileia vastatrix, have
constrained the sustainable production of Arabica coffee for over 150 years. The ability of H.
vastatrix to overcome resistance in coffee cultivars and evolve new races is inexplicable for a
pathogen that supposedly only utilizes clonal reproduction. Understanding the evolutionary
complexity between H. vastatrix and its only known host, including determining how the
pathogen evolves virulence so rapidly is crucial for disease management. Achieving such goals
relies on the availability of a comprehensive and high-quality genome reference assembly. To
date, two reference genomes have been assembled and published for H. vastatrix that, while
useful, remain fragmented and do not represent chromosomal scaffolds. Here, we present a
complete scaffolded pseudochromosome-level genome resource for H. vastatrix strain 178a
(Hv178a). Our initial assembly revealed an unusually high degree of gene duplication (over
50% BUSCO basidiomycota_odb10 genes). Upon inspection, this was predominantly due to a
single scaffold that itself showed 91.9% BUSCO Completeness. Taxonomic analysis of
predicted BUSCO genes placed this scaffold in Exobasidiomycetes and suggests it is a distinct
genome, which we have named Hv178a associated fungal genome (Hv178a AFG). The high
depth of coverage and close association with Hv178a raises the prospect of symbiosis, although
we cannot completely rule out contamination at this time. The main Ca. 546 Mbp Hv178a
genome was primarily (97.7%) localised to 11 pseudochromosomes (51.5 Mb N50), building
the foundation for future advanced studies of genome structure and organization.
Descrição
Palavras-chave
coffee rust Hv178a coffea arabica plant pathogen
Contexto Educativo
Citação
Tobias, P.A., Edwards, R.J., Surana, P., Mangelson H., Inácio V., Silva, M.C., Várzea, V., Park, R.F., Batista, D. A chromosome-level genome resource for studying virulence mechanisms and evolution of the coffee rust pathogen Hemileia vastatrix. bioRxiv, 2022.07.29.502101
Editora
bioRxiv