Logo do repositório
 
Publicação

Intra-specific variation in pea responses to ammonium nutrition leads to different degrees of tolerance

2011-02Artigo científico Acesso restrito
Ver registo simples
dc.contributor.authorCruz, Cristina
dc.contributor.authorDomínguez-Valdivia, María Dolores
dc.contributor.authorAparicio-Tejo, Pedro María
dc.contributor.authorLamsfus, Carmen
dc.contributor.authorBio, Ana
dc.contributor.authorMartins-Loução, Maria Amélia
dc.contributor.authorMoranc, Jose Fernando
dc.date.accessioned2011-09-13T16:13:33Z
dc.date.available2011-09-13T16:13:33Z
dc.date.issued2011-02
dc.descriptionACESSO via B-on: http://dx.doi.org/ doi:10.1016/j.envexpbot.2010.09.014
dc.description.abstractThe form of nitrogen nutrition affects many biochemical and physiological processes in plants, leading to markedly different growth responses. Many plant species have been characterized asammoniumtolerant or sensitive. The objective of this work was to assess the range of physiological adaptative responses involved in tolerance of ammonium nutrition at the intra-specific level. Pisum sativum L. was selected as a model of tolerance for the study, and four varieties of agricultural importance (Snap-pea, Rondo, Tristar and Eclipse) were grown hydroponically withammoniumor nitrate (0.5, 2.5, 5.0 and 10.0mM)as the sole nitrogen source. Physiological parameters including photosynthesis, respiration and ammonium contents were analyzed. Activities/expression of key enzymes of the nitrogen metabolism was also determined, for which novel antibodies against glutamate dehydrogenase and glutamine synthetase were obtained. Snap-pea was considered a reference plant for ammonium tolerance, since its biomass accumulation was not affected by nitrogen source. It avoided the physiological effects of ammonium through low growth potential, high respiratory rates and activity of the alternative pathway. A multivariate analysis of the results showed that each of the four varieties had distinct adaptative responses to ammonium nutrition, highlighting the flexibility of response to ammonium nutrition at the species level. A general activation of glutamate dehydrogenase (GDH) was observed, regardless of the degree of tolerance to ammonium, which may be due to GDH being part of a common stress response to carbon deficit rather than an ammonium tolerance mechanism.por
dc.identifier.citationEnvironmental and Experimental Botany 70 (2011) 233–243por
dc.identifier.issn0098-8472
dc.identifier.urihttp://dx.doi.org/ doi:10.1016/j.envexpbot.2010.09.014
dc.identifier.urihttp://hdl.handle.net/10451/4093
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevier
dc.subjectAmmonium nutritionpor
dc.subjectAmmonium tolerancepor
dc.subjectAmmonium stresspor
dc.subjectGlutamate dehydrogenasepor
dc.subjectGlutamine synthetasepor
dc.subjectPisum sativum L.por
dc.titleIntra-specific variation in pea responses to ammonium nutrition leads to different degrees of tolerancepor
dc.typejournal article
dspace.entity.typePublication
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
00988472_2011_233_243.pdf
Tamanho:
950.4 KB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

FC - Artigos em Revistas Internacionais