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Myeloperoxidase inhibition decreases the expression of collagen and metallopeptidase in mare endometria under in vitro conditions

2021-01-16Journal article Open access
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dc.contributor.authorAmaral, Ana
dc.contributor.authorFernandes, Carina
dc.contributor.authorRebordão, Maria Rosa
dc.contributor.authorSzostek-Mioduchowska, Anna
dc.contributor.authorLukasik, Karolina
dc.contributor.authorPinto-Bravo, Pedro
dc.contributor.authorGama, Luis
dc.contributor.authorSkarzynski, Dariusz Jan
dc.contributor.authorFerreira-Dias, Graça
dc.date.accessioned2022-01-18T17:17:30Z
dc.date.available2022-01-18T17:17:30Z
dc.date.issued2021-01-16
dc.descriptionResearch Areas: Agriculture ; Veterinary Sciences ; Zoologypt_PT
dc.description.abstractABSTRACT - Neutrophils can originate neutrophil extracellular traps (NETs). Myeloperoxidase (MPO) is a peroxidase found in NETs associated to equine endometrosis and can be inhibited by 4-aminobenzoic acid hydrazide (ABAH). Metallopeptidases (MMPs) participate in extracellular matrix stability and fibrosis development. The objectives of this in vitro work were to investigate, in explants of mare’s endometrium, (i) the ABAH capacity to inhibit MPO-induced collagen type I (COL1) expression; and (ii) the action of MPO and ABAH on the expression and gelatinolytic activity of MMP-2/-9. Explants retrieved from the endometrium of mares in follicular or mid-luteal phases were treated with MPO, ABAH, or their combination, for 24 or 48 h. The qPCR analysis measured the transcription of COL1A2, MMP2, and MMP9. Western blot and zymography were performed to evaluate COL1 protein relative abundance and gelatinolytic activity of MMP-2/-9, respectively. Myeloperoxidase elevated COL1 relative protein abundance at both treatment times in follicular phase (p < 0.05). The capacity of ABAH to inhibit MPO-induced COL1 was detected in follicular phase at 48 h (p < 0.05). The gelatinolytic activity of activated MMP-2 augmented in mid-luteal phase at 24 h after MPO treatment, but it was reduced with MPO+ABAH treatment. The activity of MMP-9 active form augmented in MPO-treated explants. However, this effect was inhibited by ABAH in the follicular phase at 48 h (p < 0.05). By inhibiting the pro-fibrotic effects of MPO, it might be possible to reduce the development of endometrosis. Metallopeptidase-2 might be involved in an acute response to MPO in the mid-luteal phase, while MMP-9 might be implicated in a prolonged exposition to MPO in the follicular phase.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAmaral A, Fernandes C, Rebordão MR, Szóstek-Mioduchowska A, Lukasik K, Pinto-Bravo P, Telo da Gama L, Jan Skarzynski D, Ferreira-Dias G. 2021. Myeloperoxidase inhibition decreases the expression of collagen and metallopeptidase in mare endometria under in vitro conditions. Animals. 11(1):208. Doi: 10.3390/ani11010208pt_PT
dc.identifier.doi10.3390/ani11010208pt_PT
dc.identifier.issn2076-2615
dc.identifier.urihttp://hdl.handle.net/10400.5/23133
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPPN/BIL/2018/1/00250/U/0001pt_PT
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relationNeutrophil extracellular traps and fibrotic cytokines: a challenge in endometrium fibrosis
dc.relation.publisherversionhttps://www.mdpi.com/2076-2615/11/1/208pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEndometrosispt_PT
dc.subjectMyeloperoxidasept_PT
dc.subject4-aminobenzoic acid hydrazidept_PT
dc.subjectFibrosispt_PT
dc.subjectMetallopeptidasespt_PT
dc.titleMyeloperoxidase inhibition decreases the expression of collagen and metallopeptidase in mare endometria under in vitro conditionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardTitleNeutrophil extracellular traps and fibrotic cytokines: a challenge in endometrium fibrosis
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT-REP%2F4202%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F101058%2F2014/PT
oaire.citation.conferencePlaceBasel, Switzerlandpt_PT
oaire.citation.titleAnimalspt_PT
oaire.citation.volumev. 11(1):208pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
person.familyNameAmaral
person.familyNameRebordão Cordeiro Simões Crisóstomo
person.familyNameGama
person.familyNameLeitão Ferreira-Dias
person.givenNameAna
person.givenNameMaria Rosa
person.givenNameLuis
person.givenNameGraça Maria
person.identifier.ciencia-idEB18-9420-421B
person.identifier.ciencia-id9515-257A-B35D
person.identifier.ciencia-idC71D-140F-E876
person.identifier.ciencia-idF51C-7F45-C720
person.identifier.orcid0000-0002-0075-262X
person.identifier.orcid0000-0002-4389-445X
person.identifier.orcid0000-0003-3894-3488
person.identifier.orcid0000-0003-0622-6513
person.identifier.ridN-4172-2018
person.identifier.scopus-author-id56394000500
person.identifier.scopus-author-id36027387300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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