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Myeloperoxidase inhibition decreases the expression of collagen and metallopeptidase in mare endometria under in vitro conditions
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Advisor(s)
Abstract(s)
ABSTRACT - Neutrophils can originate neutrophil extracellular traps (NETs). Myeloperoxidase (MPO) is
a peroxidase found in NETs associated to equine endometrosis and can be inhibited by 4-aminobenzoic
acid hydrazide (ABAH). Metallopeptidases (MMPs) participate in extracellular matrix stability and
fibrosis development. The objectives of this in vitro work were to investigate, in explants of mare’s
endometrium, (i) the ABAH capacity to inhibit MPO-induced collagen type I (COL1) expression;
and (ii) the action of MPO and ABAH on the expression and gelatinolytic activity of MMP-2/-9.
Explants retrieved from the endometrium of mares in follicular or mid-luteal phases were treated
with MPO, ABAH, or their combination, for 24 or 48 h. The qPCR analysis measured the transcription
of COL1A2, MMP2, and MMP9. Western blot and zymography were performed to evaluate COL1
protein relative abundance and gelatinolytic activity of MMP-2/-9, respectively. Myeloperoxidase
elevated COL1 relative protein abundance at both treatment times in follicular phase (p < 0.05). The
capacity of ABAH to inhibit MPO-induced COL1 was detected in follicular phase at 48 h (p < 0.05).
The gelatinolytic activity of activated MMP-2 augmented in mid-luteal phase at 24 h after MPO
treatment, but it was reduced with MPO+ABAH treatment. The activity of MMP-9 active form
augmented in MPO-treated explants. However, this effect was inhibited by ABAH in the follicular
phase at 48 h (p < 0.05). By inhibiting the pro-fibrotic effects of MPO, it might be possible to reduce
the development of endometrosis. Metallopeptidase-2 might be involved in an acute response to
MPO in the mid-luteal phase, while MMP-9 might be implicated in a prolonged exposition to MPO
in the follicular phase.
Description
Research Areas: Agriculture ; Veterinary Sciences ; Zoology
Keywords
Endometrosis Myeloperoxidase 4-aminobenzoic acid hydrazide Fibrosis Metallopeptidases
Pedagogical Context
Citation
Amaral A, Fernandes C, Rebordão MR, Szóstek-Mioduchowska A, Lukasik K, Pinto-Bravo P, Telo da Gama L, Jan Skarzynski D, Ferreira-Dias G. 2021. Myeloperoxidase inhibition decreases the expression of collagen and metallopeptidase in mare endometria under in vitro conditions. Animals. 11(1):208. Doi: 10.3390/ani11010208
Publisher
MDPI