Freeze‑dried Nannochloropsis oceanica biomass protects eicosapentaenoic acid (EPA) from metabolization in the rumen of lambs

Vitor, Ana C. M.; Francisco, Alexandra Eduarda; Silva, Joana; Pinho, Mário; Huws, Sharon A.; Santos-Silva, José; Bessa, R.J.B.; Alves, Susana P.

http://hdl.handle.net/10400.5/24005

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Autores

Vitor, Ana C. M.

Francisco, Alexandra Eduarda

Resumo(s)

Eicosapentaenoic acid (EPA) from freeze-dried biomass of Nannochloropsis oceanica microalgae resists ruminal biohydrogenation in vitro, but in vivo demonstration is needed. Therefore, the present study was designed to test the rumen protective effects of N. oceanica in lambs. Twenty-eight lambs were assigned to one of four diets: Control (C); and C diets supplemented with: 1.2% Nannochloropsis sp. oil (O); 12.3% spray-dried N. oceanica (SD); or 9.2% N. oceanica (FD), to achieve 3 g EPA /kg dry matter. Lambs were slaughtered after 3 weeks and digestive contents and ruminal wall samples were collected. EPA concentration in the rumen of lambs fed FD was about 50% higher than lambs fed SD or O diets. Nevertheless, the high levels of EPA in cecum and faeces of animals fed N. oceanica biomass, independently of the drying method, suggests that EPA was not completely released and absorbed in the small intestine. Furthermore, supplementation with EPA sources also affected the ruminal biohydrogenation of C18 fatty acids, mitigating the shift from the t10 biohydrogenation pathways to the t11 pathways compared to the Control diet. Overall, our results demonstrate that FD N. oceanica biomass is a natural rumen-protected source of EPA to ruminants.

Descrição

Research Areas: Science & Technology ; Other Topics

Palavras-chave

Polyunsaturated fatty-acids fish-oil In vitro Meat quality biohydrogenation supplementation Diet accumulation Steers Beef

URI

http://hdl.handle.net/10400.5/24005

Citação

Vitor ACM, Francisco AE, Silva J, Pinho M, Huws SA, Santos-Silva J, Bessa RJB, Alves SP. 2021. Freeze‑dried Nannochloropsis oceanica biomass protects eicosapentaenoic acid (EPA) from metabolization in the rumen of lambs. Scientific Reports, 11(1):21878. DOI 10.1038/s41598-021-01255-w