Repository logo
 
Publication

Interaction between cannabinoid type 1 and type 2 receptors in the modulation of subventricular zone and dentate Gyrus neurogenesis

2017Journal article Open access
Simple item page
dc.contributor.authorRodrigues, Rui S.
dc.contributor.authorRibeiro, Filipa
dc.contributor.authorFerreira, Filipa
dc.contributor.authorVaz, Sandra H.
dc.contributor.authorSebastião, Ana M
dc.contributor.authorXapelli, Sara
dc.date.accessioned2021-07-26T14:55:59Z
dc.date.available2021-07-26T14:55:59Z
dc.date.issued2017
dc.descriptionCopyright © 2017 Rodrigues, Ribeiro, Ferreira, Vaz, Sebastião and Xapelli. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.pt_PT
dc.description.abstractNeurogenesis in the adult mammalian brain occurs mainly in two neurogenic niches, the subventricular zone (SVZ) and the subgranular zone (SGZ) of the dentate gyrus (DG). Cannabinoid type 1 and 2 receptors (CB1R and CB2R) have been shown to differently modulate neurogenesis. However, low attention has been given to the interaction between CB1R and CB2R in modulating postnatal neurogenesis (proliferation, neuronal differentiation and maturation). We focused on a putative crosstalk between CB1R and CB2R to modulate neurogenesis and cultured SVZ and DG stem/progenitor cells from early postnatal (P1-3) Sprague-Dawley rats. Data showed that the non-selective cannabinoid receptor agonist WIN55,212-2 promotes DG cell proliferation (measured by BrdU staining), an effect blocked by either CB1R or CB2R selective antagonists. Experiments with selective agonists showed that facilitation of DG cell proliferation requires co-activation of both CB1R and CB2R. Cell proliferation in the SVZ was not affected by the non-selective receptor agonist, but it was enhanced by CB1R selective activation. However, either CB1R or CB2R selective antagonists abolished the effect of the CB1R agonist in SVZ cell proliferation. Neuronal differentiation (measured by immunocytochemistry against neuronal markers of different stages and calcium imaging) was facilitated by WIN55,212-2 at both SVZ and DG. This effect was mimicked by either CB1R or CB2R selective agonists and blocked by either CB1R or CB2R selective antagonists, cross-antagonism being evident. In summary, our findings indicate a tight interaction between CB1R and CB2R to modulate neurogenesis in the two major neurogenic niches, thus contributing to further unraveling the mechanisms behind the action of endocannabinoids in the brain.pt_PT
dc.description.sponsorshipThis work was supported by LISBOA-01-0145-FEDER-007391, project co-funded by FEDER through POR Lisboa 2020 (Programa Operacional Regional de Lisboa) from PORTUGAL 2020, and by Fundação para a Ciência e a Tecnologia (FCT). AS thanks the following supports: PTDC/DTP-FTO/3346/2014 from FCT and H2020 Twinning Action from EU (SynaNet 692340). SX is grateful for the support by the COST action BM1402. RR (IMM/BI/42-2016), FR (SFRH/BD/74662/2010), SV (SFRH/BPD/81627/2011), and SX (SFRH/BPD/76642/2011 and IF/01227/2015) were in receipt of a fellowship from FCT.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFront Pharmacol. 2017 Aug 10;8:516.pt_PT
dc.identifier.doi10.3389/fphar.2017.00516pt_PT
dc.identifier.eissn1663-9812
dc.identifier.urihttp://hdl.handle.net/10451/49125
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontierspt_PT
dc.relationLISBOA-01-0145-FEDER-007391pt_PT
dc.relationSynaptic mechanisms involved in the brain cannabinoid actions and their modulation by adenosine receptors: implications for memory and mood control
dc.relationNeurologic and Psychiatric Disorders: from synapses to networks
dc.relationADENOSINE A2A RECEPTOR MODULATION OF NEURONAL MATURATION
dc.relationMODULATION OF THE BIDIRECTIONAL COMMUNICATION BETWEEN NEURONS AND ASTROCYTES AT THE SYNAPSE: INFLUENCE OF BDNF AND P2 RECEPTORS
dc.relationENDOCANNABINOIDS AS MODULATORS OF NEUROGENESIS AND OLIGODENDROGENESIS: ROLE OF HEMOGLOBIN-DERIVED PEPTIDES
dc.relation.publisherversionhttps://www.frontiersin.org/journals/pharmacologypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCannabinoid type 1 receptorpt_PT
dc.subjectCannabinoid type 2 receptorpt_PT
dc.subjectDentate gyruspt_PT
dc.subjectPostnatal neurogenesispt_PT
dc.subjectSubventricular zonept_PT
dc.titleInteraction between cannabinoid type 1 and type 2 receptors in the modulation of subventricular zone and dentate Gyrus neurogenesispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSynaptic mechanisms involved in the brain cannabinoid actions and their modulation by adenosine receptors: implications for memory and mood control
oaire.awardTitleNeurologic and Psychiatric Disorders: from synapses to networks
oaire.awardTitleADENOSINE A2A RECEPTOR MODULATION OF NEURONAL MATURATION
oaire.awardTitleMODULATION OF THE BIDIRECTIONAL COMMUNICATION BETWEEN NEURONS AND ASTROCYTES AT THE SYNAPSE: INFLUENCE OF BDNF AND P2 RECEPTORS
oaire.awardTitleENDOCANNABINOIDS AS MODULATORS OF NEUROGENESIS AND OLIGODENDROGENESIS: ROLE OF HEMOGLOBIN-DERIVED PEPTIDES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FDTP-FTO%2F3346%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/692340/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F74662%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F81627%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBPD%2F76642%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F01227%2F2015%2FCP1317%2FCT0001/PT
oaire.citation.titleFrontiers in Pharmacologypt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamH2020
oaire.fundingStreamOE
oaire.fundingStreamInvestigador FCT
person.familyNameRodrigues
person.familyNameRibeiro
person.familyNameHenriques Vaz
person.familyNameSebastião
person.familyNameXapelli
person.givenNameRui S
person.givenNameFilipa
person.givenNameSandra Cristina
person.givenNameAna M
person.givenNameSara
person.identifier129383
person.identifier.ciencia-id6916-485D-36C3
person.identifier.ciencia-id0E1C-952D-61BE
person.identifier.ciencia-idF112-55E8-E37E
person.identifier.ciencia-id7110-C8C3-ED42
person.identifier.orcid0000-0002-9343-745X
person.identifier.orcid0000-0002-4006-7328
person.identifier.orcid0000-0003-4258-9397
person.identifier.orcid0000-0001-9030-6115
person.identifier.orcid0000-0001-6854-2509
person.identifier.ridS-1079-2018
person.identifier.scopus-author-id7004409879
person.identifier.scopus-author-id8721560200
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication80c7b611-e2d2-4481-9468-0bcb1bc8095c
relation.isAuthorOfPublication45abc672-83ac-42b0-97d5-06f5633530f8
relation.isAuthorOfPublicationb282e0fa-69eb-4079-8752-b7dd914f7745
relation.isAuthorOfPublication304abd7f-071b-4447-a8a3-4aa5f0547141
relation.isAuthorOfPublicationb4bd1deb-458d-4981-bd54-46ddf93a1927
relation.isAuthorOfPublication.latestForDiscovery80c7b611-e2d2-4481-9468-0bcb1bc8095c
relation.isProjectOfPublicationef224f38-d3c7-4f75-95c0-2515f847a1e0
relation.isProjectOfPublicationa8d0a09b-e946-45d0-ac79-0b6e4fff9e49
relation.isProjectOfPublication9dadf8dd-d035-43b9-b5eb-7962e47a8379
relation.isProjectOfPublication6de556d4-f49f-470e-a41e-957bed5e0b79
relation.isProjectOfPublication8a18b2e7-3b02-4152-9ddf-21791826205d
relation.isProjectOfPublicationf7800cfa-e1ce-4954-95dd-fec35d82a5a0
relation.isProjectOfPublication.latestForDiscoveryef224f38-d3c7-4f75-95c0-2515f847a1e0

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Interaction_Cannabinoid.pdf
Size:
1.64 MB
Format:
Adobe Portable Document Format
Download

Collections

IMM - Artigos em Revistas Internacionais
FM-IFN-Artigos em Revistas Internacionais