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Enterococcus spp. from Azeitao and Nisa PDO-cheeses: surveillance for antimicrobial drug resistance

2022-01-15Artigo científico Acesso aberto
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dc.contributor.authorRocha, Patrícia A. Bastiao
dc.contributor.authorMonteiro Marques, Joana
dc.contributor.authorBARRETO, ANTONIO
dc.contributor.authorSemedo-Lemsaddek, Teresa
dc.date.accessioned2022-06-20T22:23:23Z
dc.date.available2022-06-20T22:23:23Z
dc.date.issued2022-01-15
dc.descriptionResearch Areas: Food Science & Technologypt_PT
dc.description.abstractABSTRACT - Enterococcus spp. were isolated from PDO-cheese of Azeitão and Nisa at six cheesemaking units (Azeitão: A1, A2, A3, A4; and Nisa: N9, N10), over four years (2016–2019). Genomic typing was performed using RAPD and distinct enterococci (n = 145) were identified at the species level by multiplex-PCR and evaluated regarding antimicrobial drug resistance (AMR). Antibiotics from nine distinct classes (aminoglycosides, macrolides, oxazolidinones, chloramphenicol, streptogramins, tetracyclines, glycopeptides, β-lactams, and quinolones) were selected for AMR surveillance and breakpoint criteria defined by EUCAST and CLSI were considered and compared. Regarding species allocation, 78 enterococci were identified as E. faecium, 37 confirmed as E. faecalis and 30 as E. durans. High levels of resistance to quinupristin-dalfopristin, tetracycline and teicoplanin were observed. Some resistances to clinically relevant antimicrobials were also detected, including β-lactams, aminoglycosides, and glycopeptides. Two isolates were considered multidrug-resistant, one according to EUCAST and the other to CLSI breakpoint criteria. Overall, considering the absence of reports regarding enterococcalrelated toxinfections or infections resulting from the consumption of PDO-cheeses, traditional foods harbouring these bacteria should be considered safe. However, the possibility of horizontal gene transfer events associated with antibiotic resistance determinants further highlights the importance for AMR surveillance along the food chain.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRocha PAB, Marques JMM, Barreto AS, Semedo-Lemsaddek T. 2022. Enterococcus spp. from Azeitao and Nisa PDO-cheeses: surveillance for antimicrobial drug resistance. LWT-Food Science and Technology, 154:112622. DOI: https://doi.org/10.1016/j.lwt.2021.112622pt_PT
dc.identifier.doi10.1016/j.lwt.2021.112622pt_PT
dc.identifier.eissn1096-1127
dc.identifier.issn0023-6438
dc.identifier.urihttp://hdl.handle.net/10400.5/24592
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationDL57/2016/CP1438/CT0004pt_PT
dc.relationauthEntic cheesoMics: from big data, through labOratory, towards the fuTure of protected desIgnation of OrigiN
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0023643821017758?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEnterococcus spp.pt_PT
dc.subjectPDO-Cheesept_PT
dc.subjectAntimicrobial resistancept_PT
dc.subjectFood chainpt_PT
dc.subjectSurveillancept_PT
dc.titleEnterococcus spp. from Azeitao and Nisa PDO-cheeses: surveillance for antimicrobial drug resistancept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPTDC/OCE-ETA/1785/2020
oaire.awardNumberUIDB/00276/2020
oaire.awardTitleauthEntic cheesoMics: from big data, through labOratory, towards the fuTure of protected desIgnation of OrigiN
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FOCE-ETA%2F1785%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.citation.conferencePlaceNetherlandspt_PT
oaire.citation.issue112622pt_PT
oaire.citation.titleLWT-Food Science and Technologypt_PT
oaire.citation.volume154pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMonteiro Marques
person.familyNameBARRETO
person.familyNameSemedo-Lemsaddek
person.givenNameJoana
person.givenNameANTONIO SALVADOR FERREIRA HENRIQUES BARRETO
person.givenNameTeresa
person.identifier.ciencia-id581C-9E81-6562
person.identifier.ciencia-idF713-6444-D446
person.identifier.ciencia-idA819-03CE-B03E
person.identifier.orcid0000-0001-8944-3235
person.identifier.orcid0000-0002-1679-4461
person.identifier.orcid0000-0002-8349-3802
person.identifier.ridJ-3010-2013
person.identifier.scopus-author-id25926127100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication938253cf-393b-497a-b18b-6a49c6fe9460
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