Enterococcus spp. from Azeitao and Nisa PDO-cheeses: surveillance for antimicrobial drug resistance

Rocha, Patrícia A. Bastiao; Monteiro Marques, Joana; BARRETO, ANTONIO; Semedo-Lemsaddek, Teresa

http://hdl.handle.net/10400.5/24592

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Autores

Rocha, Patrícia A. Bastiao

Monteiro Marques, Joana

BARRETO, ANTONIO

Semedo-Lemsaddek, Teresa

Resumo(s)

ABSTRACT - Enterococcus spp. were isolated from PDO-cheese of Azeitão and Nisa at six cheesemaking units (Azeitão: A1, A2, A3, A4; and Nisa: N9, N10), over four years (2016–2019). Genomic typing was performed using RAPD and distinct enterococci (n = 145) were identified at the species level by multiplex-PCR and evaluated regarding antimicrobial drug resistance (AMR). Antibiotics from nine distinct classes (aminoglycosides, macrolides, oxazolidinones, chloramphenicol, streptogramins, tetracyclines, glycopeptides, β-lactams, and quinolones) were selected for AMR surveillance and breakpoint criteria defined by EUCAST and CLSI were considered and compared. Regarding species allocation, 78 enterococci were identified as E. faecium, 37 confirmed as E. faecalis and 30 as E. durans. High levels of resistance to quinupristin-dalfopristin, tetracycline and teicoplanin were observed. Some resistances to clinically relevant antimicrobials were also detected, including β-lactams, aminoglycosides, and glycopeptides. Two isolates were considered multidrug-resistant, one according to EUCAST and the other to CLSI breakpoint criteria. Overall, considering the absence of reports regarding enterococcalrelated toxinfections or infections resulting from the consumption of PDO-cheeses, traditional foods harbouring these bacteria should be considered safe. However, the possibility of horizontal gene transfer events associated with antibiotic resistance determinants further highlights the importance for AMR surveillance along the food chain.

Descrição

Research Areas: Food Science & Technology

Palavras-chave

Enterococcus spp. PDO-Cheese Antimicrobial resistance Food chain Surveillance

URI

http://hdl.handle.net/10400.5/24592

Citação

Rocha PAB, Marques JMM, Barreto AS, Semedo-Lemsaddek T. 2022. Enterococcus spp. from Azeitao and Nisa PDO-cheeses: surveillance for antimicrobial drug resistance. LWT-Food Science and Technology, 154:112622. DOI: https://doi.org/10.1016/j.lwt.2021.112622