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Avaliação in vitro e ex vivo da eficácia antimicrobiana de nanopartículas de MgO na eliminação de Enterococcus faecalis
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Objetivos: Avaliar o potencial bactericida de uma solução de nanopartículas de óxido de magnésio (Nano-MgO) contra Enterococcus faecalis, comparativamente à irrigação convencional com uma solução de hipoclorito de sódio (NaOCl), através de um modelo in vitro e ex vivo.
Material e Métodos: Foram preparados e esterilizados 54 dentes unirradiculares. Realizou-se a preparação de uma solução de Nano-MgO (5mg/L). Foi preparada uma suspensão bacteriana da estirpe referência E. faecalis ATCC 51299. Avaliou-se preliminarmente a atividade antimicrobiana das soluções teste com recurso à técnica spot-on-lawn. Inoculou-se 10μL da suspensão de E. faecalis no interior de cada canal radicular. Após um período de incubação (37ºC, durante 24h), os dentes foram irrigados segundo o padrão de desinfeção canalar estabelecido para cada grupo experimental: no grupo 1 (n=9) testou-se uma solução comercial de hipoclorito de sódio a 5,25 %, enquanto que no grupo 2 (n=9) testou-se a solução de Nano- MgO. A análise do protocolo de desinfeção experimental foi realizada através de métodos de cultura bacteriológica, sendo que todas as alterações na turvação do meio foram avaliadas e registadas às 24, 48 e 72h. Os resultados obtidos foram analisados estatisticamente, com recurso à realização do teste exato de Fisher, com nível de significância de 5%.
Resultados: Obtiveram-se diferenças estatisticamente significativas (p<0,05) entre os resultados obtidos para os dois grupos experimentais, com a solução de Nano-MgO (5mg/L) a apresentar uma eficácia bactericida inferior relativamente à irrigação convencional com uma solução de NaOCl a 5,25%.
Conclusões: A aplicação de uma solução aquosa de Nano-MgO como irrigante canalar não é recomendada do ponto de vista clínico. Contudo, as Nano-MgO apresentam uma elevada atividade antimicrobiana in vitro contra E.faecalis, pelo que se sugere a realização de estudos futuros que avaliem a sua utilização numa outra fase do tratamento endodôntico, nomeadamente no momento de obturação canalar.
Aim: The aim of this study was to evaluate the in vitro and ex vivo bactericidal potential of nano-magnesium oxide (MgO) aqueous solution against Enterococcus faecalis, compared to conventional irrigation with a sodium hypochlorite solution (NaOCl). Material and Methods: 54 single-rooted teeth were prepared and sterilized. A Nano-MgO solution (5mg/L) was prepared. A bacterial suspension of the reference strain E. faecalis ATCC 51299 was prepared. The antimicrobial activity of the test solutions was preliminarily evaluated using spot-on-lawn technique. A 10μL suspension of E. faecalis was inoculated inside each root canal. After an incubation period (37°C, for 24h), the teeth were irrigated according to the canal disinfection protocol established for each experimental group: group 1 (n=9) tested a commercial 5.25% sodium hypochlorite solution, while group 2 (n=9) tested the Nano-MgO solution. The experimental disinfection protocol was analyzed using bacteriological culture methods. All changes in the turbidity of the culture medium were evaluated and recorded at 24, 48 and 72h. The results obtained were statistically analyzed using Fisher's exact test with a level of significance at 5%. Results: Statistically significant differences (p<0.05) were obtained between the experimental groups, with the Nano-MgO solution (5mg/L) showing a lower bactericidal efficacy compared to conventional irrigation with a 5.25% NaOCl solution. Conclusions: The application of a Nano-MgO aqueous solution as a canal irrigant is not clinically recommended. However, Nano-MgO shows a high antimicrobial activity in vitro against E. faecalis. So further investigations should be carried out to evaluate its use in other phases of the endodontic treatment, namely during canal filling.
Aim: The aim of this study was to evaluate the in vitro and ex vivo bactericidal potential of nano-magnesium oxide (MgO) aqueous solution against Enterococcus faecalis, compared to conventional irrigation with a sodium hypochlorite solution (NaOCl). Material and Methods: 54 single-rooted teeth were prepared and sterilized. A Nano-MgO solution (5mg/L) was prepared. A bacterial suspension of the reference strain E. faecalis ATCC 51299 was prepared. The antimicrobial activity of the test solutions was preliminarily evaluated using spot-on-lawn technique. A 10μL suspension of E. faecalis was inoculated inside each root canal. After an incubation period (37°C, for 24h), the teeth were irrigated according to the canal disinfection protocol established for each experimental group: group 1 (n=9) tested a commercial 5.25% sodium hypochlorite solution, while group 2 (n=9) tested the Nano-MgO solution. The experimental disinfection protocol was analyzed using bacteriological culture methods. All changes in the turbidity of the culture medium were evaluated and recorded at 24, 48 and 72h. The results obtained were statistically analyzed using Fisher's exact test with a level of significance at 5%. Results: Statistically significant differences (p<0.05) were obtained between the experimental groups, with the Nano-MgO solution (5mg/L) showing a lower bactericidal efficacy compared to conventional irrigation with a 5.25% NaOCl solution. Conclusions: The application of a Nano-MgO aqueous solution as a canal irrigant is not clinically recommended. However, Nano-MgO shows a high antimicrobial activity in vitro against E. faecalis. So further investigations should be carried out to evaluate its use in other phases of the endodontic treatment, namely during canal filling.
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Teses de mestrado - 2021 Saúde Oral