Repository logo
 
Publication

Influence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-Challenged Weaned Piglets

2021-04-16Journal article Open access
Simple item page
dc.contributor.authorPrates, José A.M
dc.contributor.authorFreire, João
dc.contributor.authorAlmeida, André
dc.contributor.authorMartins, Cátia Falcão
dc.contributor.authorRibeiro, David M.
dc.contributor.authorOsorio, Hugo
dc.contributor.authorPinho, Mário
dc.contributor.authorLopes, Paula Alexandra
dc.contributor.authorCorreia, Jorge Manuel de Jesus
dc.contributor.authorPinto, Rui M. A.
dc.date.accessioned2022-02-17T21:50:15Z
dc.date.available2022-02-17T21:50:15Z
dc.date.issued2021-04-16
dc.descriptionResearch Areas: Agriculture ; Veterinary Sciences ; Zoologypt_PT
dc.description.abstractIn order to investigate the effect of a dietary amino acid mixture supplementation in lipopolysaccharide (LPS)-challenged weaned piglets, twenty-seven 28-day-old (8.2 ± 1.0 kg) newly weaned piglets were randomly allocated to one of three experimental treatments for five weeks. Diet 1: a CTRL treatment. Diet 2: an LPS treatment, where piglets were intraperitoneally administered LPS (25 μg/kg) on day 7. Diet 3: an LPS+MIX treatment, where piglets were intraperitoneally administered LPS on day 7 and fed a diet supplemented with a mixture of 0.3% of arginine, branched-chain amino acids (leucine, valine, and isoleucine), and cystine (MIX). Blood samples were drawn on day 10 and day 35, and serum was analysed for selected chemical parameters and proteomics. The LPS and LPS+MIX groups exhibited an increase in haptoglobin concentrations on day 10. The LPS group showed an increased cortisol concentration, while this concentration was reduced in the LPS+MIX group compared to the control group. Similarly, the LPS+MIX group showed a decreased haptoglobin concentration on day 35 compared to the two other groups. Immunoglobulin concentrations were affected by treatments. Indeed, on day 10, the concentrations of IgG and IgM were decreased by the LPS challenge, as illustrated by the lower concentrations of these two immunoglobulins in the LPS group compared to the control group. In addition, the supplementation with the amino acid mixture in the LPS+MIX further decreased IgG and increased IgM concentrations compared to the LPS group. Although a proteomics approach did not reveal important alterations in the protein profile in response to treatments, LPS-challenged piglets had an increase in proteins linked to the immune response, when compared to piglets supplemented with the amino acid mixture. Overall, data indicate that LPS-challenged piglets supplemented with this amino acid mixture are more protected against the detrimental effects of LPS.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPrates JAM, Freire JPB, de Almeida AM, Martins C, Ribeiro DM, Osorio H, Pinho MAS, Lopes PA, Correia JMJ, Pinto RMA. 2021. Influence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-Challenged Weaned Piglets. Animals, 11(4):1143. Doi: 10.3390/ani11041143pt_PT
dc.identifier.doi10.3390/ani11041143pt_PT
dc.identifier.issn2076-2615
dc.identifier.urihttp://hdl.handle.net/10400.5/23582
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationUIDB/CVT/00276/2020pt_PT
dc.relationPEST/UID/AGR/4129/2020pt_PT
dc.relationDL57/2016/CP1438/CT0007pt_PT
dc.relationInclusion of Laminaria and Ulva seaweeds as a feedstuff in piglet diets: effect on animal performance and physiology
dc.relation.publisherversionhttps://www.mdpi.com/2076-2615/11/4/1143pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPigletspt_PT
dc.subjectAmino acidspt_PT
dc.subjectImmunitypt_PT
dc.subjectChallengept_PT
dc.titleInfluence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-Challenged Weaned Pigletspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInclusion of Laminaria and Ulva seaweeds as a feedstuff in piglet diets: effect on animal performance and physiology
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F143992%2F2019/PT
oaire.citation.conferencePlaceBasel, Switzerlandpt_PT
oaire.citation.titleAnimalspt_PT
oaire.citation.volumeVol. 11(4):1143pt_PT
person.familyNameMestre Prates
person.familyNameBengala Freire
person.familyNameMartinho de Almeida
person.familyNameFalcão Martins
person.familyNameSoares de Pinho
person.familyNameLopes
person.familyNameJesus Correia
person.givenNameJosé António
person.givenNameJoão Pedro
person.givenNameAndré
person.givenNameCátia
person.givenNameMário
person.givenNamePaula Alexandra
person.givenNameJorge Manuel
person.identifier.ciencia-id2617-53FF-04CD
person.identifier.ciencia-id871A-F6C8-60FE
person.identifier.ciencia-id1E14-92CC-B163
person.identifier.ciencia-id5A14-15B0-2682
person.identifier.ciencia-id2719-4714-9EC1
person.identifier.ciencia-id0912-38D0-9D20
person.identifier.ciencia-id7311-54F2-A545
person.identifier.orcid0000-0003-1032-5987
person.identifier.orcid0000-0002-1811-4378
person.identifier.orcid0000-0002-7810-3988
person.identifier.orcid0000-0002-1017-3269
person.identifier.orcid0000-0002-6755-4572
person.identifier.orcid0000-0002-1909-4540
person.identifier.ridK-9934-2013
person.identifier.scopus-author-id56003169500
person.identifier.scopus-author-id7202364133
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationc5489c57-c896-4036-8b8a-6a4192846260
relation.isAuthorOfPublication99660917-13cd-47e0-bf7d-667497ebcfbe
relation.isAuthorOfPublication722b3039-6553-4059-8e11-d5eaf113a8cd
relation.isAuthorOfPublication9e190a84-f41d-40d8-a8f2-0c4b42cd6fac
relation.isAuthorOfPublication51e027ce-1839-43b1-a09d-3db149a049dc
relation.isAuthorOfPublication3b832220-c545-47de-9db5-1e23b8c91bf1
relation.isAuthorOfPublicationbc5cb57b-d601-4020-9adb-ee4cb0996dc7
relation.isAuthorOfPublication.latestForDiscoveryc5489c57-c896-4036-8b8a-6a4192846260
relation.isProjectOfPublicationf9a66ffe-a149-4ad9-9b82-8ebd2159996c
relation.isProjectOfPublication.latestForDiscoveryf9a66ffe-a149-4ad9-9b82-8ebd2159996c

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Influence of Dietary Supplementation with an Amino Acid Mixture on Inflammatory Markers, Immune Status and Serum Proteome in LPS-Challenged Weaned Piglets.pdf
Size:
1.18 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIISA - Artigos em revistas internacionais