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Abstract(s)
Este ensaio insere-se numa metodologia de estudo de caso e a amostra inclui 106 fêmeas selecionadas entre as vacas Holstein-Frísias múltiparas. A recolha dos dados iniciou-se a seguir ao parto com a medição da concentração sanguínea da proteína total (PT). Posteriormente, no quinto dia após o parto, foi efetuada uma segunda medição da concentração sanguínea da PT. As vacas leiteiras foram seguidas até 30 dias após o parto e incorporadas nos grupos sem e com doença pós-parto, “paratuberculose +” sem e com sintomas da doença pós-parto. Foram consideradas as principais e mais frequentes doenças do pós-parto em vacas leiteiras multíparas em regime intensivo de produção, como a retenção placentária, metrite, mastite e cetose. Procurou adotar-se uma metodologia semelhante de medição da PT sérica aos vitelos recém-nascidos a fim de se conseguir um diagnóstico precoce de doenças do pós-parto em vacas leiteiras multíparas, através do uso do refratómetro.
No total das 106 vacas multíparas 70 fêmeas não adoeceram (66%) e 36 animais adoeceram (34%) no pós-parto. A variação (VarO, g/dL) da PT medida pelo refratómetro ótico (PTo) e a variação (VarD, % de Brix) da PT medida pelo refratómetro digital (PTd) foram estatisticamente significativas entre os grupos sem e com doença pós-parto (p < 0,05), sendo que no grupo sem doença pós-parto a Var da PT sérica foi positiva e no grupo com doença pós-parto, a Var foi negativa. O decréscimo no valor da PT entre o dia do parto e o quinto dia pós-parto, sugere possivelmente um sinal de doença. Nos grupos “paratuberculose +” sem e com sintomas de doença pós-parto, a VarO (g/dL) da PTo e a VarD (% de Brix) da PTd não foram estatisticamente significativas (p > 0,05). Os dois tipos de refratómetros usados para medir a PT sérica apresentaram uma forte correlação entre si (ρ=0,98), sendo que ambas as ferramentas foram fiáveis para esse fim, quando usados em vacas leiteiras. Porém, sugere-se a realização de outros estudos, análogos, com um maior número de animais na amostra, sobretudo dentro do grupo de cada doença do pós-parto. Seria igualmente importante fazer as medições da PT sérica também nas vacas secas, antes do parto (cerca de três semanas e uma semana antes da data prevista do parto) de forma a poder verificar a diminuição ou não da PT sérica à medida que se aproxima o parto como uma possível consequência da imunossupressão presente neste período.
ABSTRACT - MEASUREMENT OF TOTAL PROTEIN IN RECENTLY CALVING HOLSTEIN-FRIESIAN COWS AND THEIR POINT USE IN CLINICAL PRACTICE - The study is part of a case study methodology and the sample includes 106 females selected from the multiparous Holstein Friesian cows. Data collection began after calving by measuring total protein (PT) blood concentration. Subsequently, on the fifth day after calving, a second measurement of PT blood concentration was performed. Dairy cows were followed up to 30 days postpartum and incorporated into the groups without and with postpartum disease, “paratuberculosis +” no and with symptoms of postpartum disease. They were considered the major and more frequent postpartum diseases in multiparous cows in production intensively, such as retained placenta, metritis, mastitis and ketosis. An attempt was made to adopt a methodology for measuring serum PT in newborn calves for the early diagnosis of postpartum diseases in multiparous dairy cows. In the total 106 multiparous cows 70 animals did not become ill (66%) and 36 animals became ill (34%). The variation (VarO, g / dL) of the total protein measured by the optical refractometer (PTo) and the variation (VarD,% of Brix) of the total ptotein measured by the digital refractometer (PTd) were statistically significant between groups without and with postpartum disease (p <0.05) (p <0.05), and in the group without postpartum disease the Var of serum PT was positive and negative in the group with postpartum disease.The decrease in the PT value between the day of calving and fifth day pospartum possibly indicates disease. In the “paratuberculosis +” group the VarO (g/dL) of PTo and the variation (VarD) of PTd were not statistically significant (p > 0.05). Both types of refractometers used to measure serum PT were strongly correlated (ρ = 0.98), and both tools were reliable for this purpose when used in dairy cows. However, other similar studies should be performed with a larger number of animals sampled, even within the group of each postpartum disease. It would also be important to measure serum PT in dry cows before calving (about three weeks and one week before the expected date of calving) in order to be able to check the decrease or not of serum PT as calving approaches as a possible consequence of immunosuppression present in this period.
ABSTRACT - MEASUREMENT OF TOTAL PROTEIN IN RECENTLY CALVING HOLSTEIN-FRIESIAN COWS AND THEIR POINT USE IN CLINICAL PRACTICE - The study is part of a case study methodology and the sample includes 106 females selected from the multiparous Holstein Friesian cows. Data collection began after calving by measuring total protein (PT) blood concentration. Subsequently, on the fifth day after calving, a second measurement of PT blood concentration was performed. Dairy cows were followed up to 30 days postpartum and incorporated into the groups without and with postpartum disease, “paratuberculosis +” no and with symptoms of postpartum disease. They were considered the major and more frequent postpartum diseases in multiparous cows in production intensively, such as retained placenta, metritis, mastitis and ketosis. An attempt was made to adopt a methodology for measuring serum PT in newborn calves for the early diagnosis of postpartum diseases in multiparous dairy cows. In the total 106 multiparous cows 70 animals did not become ill (66%) and 36 animals became ill (34%). The variation (VarO, g / dL) of the total protein measured by the optical refractometer (PTo) and the variation (VarD,% of Brix) of the total ptotein measured by the digital refractometer (PTd) were statistically significant between groups without and with postpartum disease (p <0.05) (p <0.05), and in the group without postpartum disease the Var of serum PT was positive and negative in the group with postpartum disease.The decrease in the PT value between the day of calving and fifth day pospartum possibly indicates disease. In the “paratuberculosis +” group the VarO (g/dL) of PTo and the variation (VarD) of PTd were not statistically significant (p > 0.05). Both types of refractometers used to measure serum PT were strongly correlated (ρ = 0.98), and both tools were reliable for this purpose when used in dairy cows. However, other similar studies should be performed with a larger number of animals sampled, even within the group of each postpartum disease. It would also be important to measure serum PT in dry cows before calving (about three weeks and one week before the expected date of calving) in order to be able to check the decrease or not of serum PT as calving approaches as a possible consequence of immunosuppression present in this period.
Description
Dissertação de Mestrado Integrado em Medicina Veterinária
Keywords
vaca leiteira proteína total imunossupressão periparto refratómetro doenças do pós-parto dairy cow total protein peripartum immunosuppression refractometer postpartum disease
Pedagogical Context
Citation
Bystrova SI 2019. Medição da proteína total em vacas holstein-frísias recém-paridas e sua pontual utilização na prática clínica. [dissertação de mestrado]. Lisboa: FMV-Universidade de Lisboa.
Publisher
Universidade de Lisboa, Faculdade de Medicina Veterinária