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Polymicrobial biofilms by diabetic foot clinical isolates

2015Journal article Restricted access
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dc.contributor.authorMottola, Carla
dc.contributor.authorMendes, João J.
dc.contributor.authorCristino, José Melo
dc.contributor.authorCavaco Silva, Patrícia
dc.contributor.authorTavares, Luis
dc.contributor.authorOliveira, Manuela
dc.date.accessioned2023-01-09T12:36:57Z
dc.date.available2023-01-09T12:36:57Z
dc.date.issued2015
dc.description© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2015pt_PT
dc.description.abstractDiabetes mellitus is a major chronic disease that continues to increase significantly. One of the most important and costly complications of diabetes is foot ulceration that may be colonized by pathogenic and antimicrobial resistant bacteria, which may express several virulence factors that could impair treatment success. These bacterial communities can be organized in polymicrobial biofilms, which may be responsible for diabetic foot ulcer (DFU) chronicity. We evaluated the influence of polymicrobial communities in the ability of DFU isolates to produce biofilm, using a microtiter plate assay and a multiplex fluorescent in situ hybridization, at three time points (24, 48, 72 h), after evaluating biofilm formation by 95 DFU isolates belonging to several bacterial genera (Staphylococcus, Corynebacterium, Enterococcus, Pseudomonas and Acinetobacter). All isolates were biofilm-positive at 24 h, and the amount of biofilm produced increased with incubation time. Pseudomonas presented the higher biofilm production, followed by Corynebacterium, Acinetobacter, Staphylococcus and Enterococcus. Significant differences were found in biofilm formation between the three time points. Polymicrobial communities produced higher biofilm values than individual species. Pseudomonas + Enterococcus, Acinetobacter + Staphylococcus and Corynebacterium + Staphylococcus produced higher biofilm than the ones formed by E. faecalis + Staphylococcus and E. faecalis + Corynebacterium. Synergy between bacteria present in dual or multispecies biofilms has been described, and this work represents the first report on time course of biofilm formation by polymicrobial communities from DFUs including several species. The biological behavior of different bacterial species in polymicrobial biofilms has important clinical implications for the successful treatment of these infections.pt_PT
dc.description.sponsorshipThis study was conducted with the financial support of the Foundation for Science and Technology (FCT Project PTDC/SAU-MIC/122816/2010-Biofilms in diabetic foot: microbial virulence characterization and cross-talk of major isolates). Carla Mottola holds a PhD fellowship (SFRH/BD/72872/2010) from FCT, Portugalpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFolia Microbiol (Praha). 2016 Jan;61(1):35-43pt_PT
dc.identifier.doi10.1007/s12223-015-0401-3pt_PT
dc.identifier.eissn1874-9356
dc.identifier.issn0015-5632
dc.identifier.urihttp://hdl.handle.net/10451/55720
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.relationBACTERIAL CROSS-TALK IN BIOFILM-ENCLOSED MIXED POPULATIONS FROM DIABETIC FOOT INFECTION
dc.relation.publisherversionhttps://www.springer.com/journal/12223pt_PT
dc.titlePolymicrobial biofilms by diabetic foot clinical isolatespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBACTERIAL CROSS-TALK IN BIOFILM-ENCLOSED MIXED POPULATIONS FROM DIABETIC FOOT INFECTION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-MIC%2F122816%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F72872%2F2010/PT
oaire.citation.endPage43pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage35pt_PT
oaire.citation.titleFolia Microbiologicapt_PT
oaire.citation.volume61pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameMottola
person.familyNameMelo Cristino
person.familyNameMorgado Tavares
person.familyNameOliveira
person.givenNameCarla
person.givenNameJosé
person.givenNameLuis Manuel
person.givenNameManuela
person.identifier.ciencia-id871E-6AD6-F37C
person.identifier.ciencia-id9011-18C6-482D
person.identifier.ciencia-idBE1E-2B3B-92D2
person.identifier.orcid0000-0002-3676-9191
person.identifier.orcid0000-0001-8643-1722
person.identifier.orcid0000-0001-8671-6285
person.identifier.orcid0000-0002-3910-1525
person.identifier.ridH-3726-2013
person.identifier.scopus-author-id7004053640
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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