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Cellular and substrate-specific interactions characterising some of the esca pathogens of grapevine
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Autores
Orientador(es)
Resumo(s)
Esca is a grapevine trunk disease caused by one association of fungi. Phaeomoniella
chlamydospora is presumably the first fungus to infect grapevine. Another pathogen
which belongs to the fungal complex is Fomitiporia mediterranea, responsible for the
final wood decay process. In this study Trametes versicolor was used as a control
because it is capable of degrading the wood, and is not usually associated with esca
disease. The experiments were done with wood from different plant species, namely
Vitis vinifera, Prunus sp. and Ficus carica. The latter was used to investigate a possible
antifungal activity.
When subjected to anaerobic conditions, the three fungi do not present any growth.
The fungi produce reactive oxygen species (ROS) when they interact in culture. The
interaction between P. chlamydospora and T. versicolor was the most relevant. All
fungi were able to degrade the wood of the different plant species analysed, as
evaluated by microscopy and staining techniques. The wood was also used to prepare
different culture media, based on wood-powder agar and wood-extract agar. For each
case, different growth rates were obtained. The peripheral growth of the hyphae was
followed in the different media and no alterations of the phenotype were found, except
for the length of the hyphae
Descrição
Mestrado em Biologia funcional - Instituto Superior de Agronomia
Palavras-chave
grapevine esca Phaemoniella chamydospora wood degradation
Contexto Educativo
Citação
Oliveira, João Daniel Condeço - Cellular and substrate-specific interactions characterising some of the esca pathogens of grapevine. Lisboa: ISA, 2011