Repository logo
 
Publication

Deflamin Attenuated Lung Tissue Damage in an Ozone-Induced COPD Murine Model by Regulating MMP-9 Catalytic Activity

2024-05Journal article Open access
Simple item page
dc.contributor.authorBaltazar-García, Elia Ana
dc.contributor.authorVargas-Guerrero, Belinda
dc.contributor.authorLima, Ana
dc.contributor.authorFerreira, Ricardo Boavida
dc.contributor.authorMendoza-Magaña, María Luisa
dc.contributor.authorRamírez-Herrera, Mario Alberto
dc.contributor.authorBaltazar-Díaz, Tonatiuh Abimael
dc.contributor.authorDomínguez-Rosales, José Alfredo
dc.contributor.authorSalazar-Montes, Adriana María
dc.contributor.authorGurrola-Díaz, Carmen Magdalena
dc.date.accessioned2025-07-24T15:03:16Z
dc.date.available2025-07-24T15:03:16Z
dc.date.issued2024-05
dc.description.abstractChronic obstructive pulmonary disease (COPD) is comprised of histopathological alter- ations such as pulmonary emphysema and peribronchial fibrosis. Matrix metalloproteinase 9 (MMP-9) is one of the key enzymes involved in both types of tissue remodeling during the development of lung damage. In recent studies, it was demonstrated that deflamin, a protein component extracted from Lupinus albus, markedly inhibits the catalytic activity of MMP-9 in experimental models of colon adenocarcinoma and ulcerative colitis. Therefore, in the present study, we investigated for the first time the biological effect of deflamin in a murine COPD model induced by chronic exposure to ozone. Ozone exposure was carried out in C57BL/6 mice twice a week for six weeks for 3 h each time, and the treated group was orally administered deflamin (20 mg/kg body weight) after each ozone exposure. The histological results showed that deflamin attenuated pulmonary emphysema and peribronchial fibrosis, as evidenced by H&E and Masson’s trichrome staining. Furthermore, deflamin administration significantly decreased MMP-9 activity, as assessed by fluorogenic substrate assay and gelatin zymography. Interestingly, bioinformatic analysis reveals a plausible interaction between deflamin and MMP-9. Collectively, our findings demonstrate the therapeutic potential of deflamin in a COPD murine model, and suggest that the attenuation of the development of lung tissue damage occurs by deflamin-regulated MMP-9 catalytic activity.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBaltazar-García, E.A.; Vargas-Guerrero, B.; Lima, A.; Boavida Ferreira, R.; Mendoza-Magaña, M.L.; Ramírez-Herrera, M.A.; Baltazar-Díaz, T.A.; Domínguez-Rosales, J.A.; Salazar-Montes, A.M.; Gurrola-Díaz, C.M. Deflamin Attenuated Lung Tissue Damage in an Ozone-Induced COPD Murine Model by Regulating MMP-9 Catalytic Activity. Int. J. Mol. Sci. 2024, 25, 5063. https://doi.org/10.3390/ijms25105063pt_PT
dc.identifier.doi10.3390/ijms25105063pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/102419
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationDevelopment of lupine and chickpea-based beverage as a functional food against colorectal cancer
dc.relation.publisherversionhttps://www.mdpi.com/journal/ijmspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectpulmonary diseasept_PT
dc.subjectlupin proteinspt_PT
dc.subjectemphysemapt_PT
dc.subjectperibronchial fibrosispt_PT
dc.subjectmatrix metalloproteinasept_PT
dc.titleDeflamin Attenuated Lung Tissue Damage in an Ozone-Induced COPD Murine Model by Regulating MMP-9 Catalytic Activitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDevelopment of lupine and chickpea-based beverage as a functional food against colorectal cancer
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de Projetos IC&DT em Todos os Domínios Científicos/PTDC%2FOCE-ETA%2F4836%2F2021/PT
oaire.citation.issue10pt_PT
oaire.citation.startPage5063pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume25pt_PT
oaire.fundingStreamConcurso de Projetos IC&DT em Todos os Domínios Científicos
person.familyNameFerreira
person.givenNameRicardo Boavida
person.identifier86030
person.identifier.ciencia-id371D-28A0-4027
person.identifier.orcid0000-0002-5027-7564
person.identifier.scopus-author-id8285312700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0f32c437-6c87-47d7-998b-c97c4695806a
relation.isAuthorOfPublication.latestForDiscovery0f32c437-6c87-47d7-998b-c97c4695806a
relation.isProjectOfPublication22823fa4-d554-4867-ba97-a3d6a72ebc96
relation.isProjectOfPublication.latestForDiscovery22823fa4-d554-4867-ba97-a3d6a72ebc96

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ijms-25-05063.pdf
Size:
6.81 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.2 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISA - Artigos em Revistas Internacionais