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Advisor(s)
Abstract(s)
Chronic obstructive pulmonary disease (COPD) is comprised of histopathological alter-
ations such as pulmonary emphysema and peribronchial fibrosis. Matrix metalloproteinase 9 (MMP-9)
is one of the key enzymes involved in both types of tissue remodeling during the development of
lung damage. In recent studies, it was demonstrated that deflamin, a protein component extracted
from Lupinus albus, markedly inhibits the catalytic activity of MMP-9 in experimental models of
colon adenocarcinoma and ulcerative colitis. Therefore, in the present study, we investigated for the
first time the biological effect of deflamin in a murine COPD model induced by chronic exposure to
ozone. Ozone exposure was carried out in C57BL/6 mice twice a week for six weeks for 3 h each
time, and the treated group was orally administered deflamin (20 mg/kg body weight) after each
ozone exposure. The histological results showed that deflamin attenuated pulmonary emphysema
and peribronchial fibrosis, as evidenced by H&E and Masson’s trichrome staining. Furthermore,
deflamin administration significantly decreased MMP-9 activity, as assessed by fluorogenic substrate
assay and gelatin zymography. Interestingly, bioinformatic analysis reveals a plausible interaction
between deflamin and MMP-9. Collectively, our findings demonstrate the therapeutic potential of
deflamin in a COPD murine model, and suggest that the attenuation of the development of lung
tissue damage occurs by deflamin-regulated MMP-9 catalytic activity.
Description
Keywords
pulmonary disease lupin proteins emphysema peribronchial fibrosis matrix metalloproteinase
Pedagogical Context
Citation
Baltazar-García, E.A.; Vargas-Guerrero, B.; Lima, A.; Boavida Ferreira, R.; Mendoza-Magaña, M.L.; Ramírez-Herrera, M.A.; Baltazar-Díaz, T.A.; Domínguez-Rosales, J.A.; Salazar-Montes, A.M.; Gurrola-Díaz, C.M. Deflamin Attenuated Lung Tissue Damage in an Ozone-Induced COPD Murine Model by Regulating MMP-9 Catalytic Activity. Int. J. Mol. Sci. 2024, 25, 5063. https://doi.org/10.3390/ijms25105063
Publisher
MDPI