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Seroprevalence of anti‐SARS‐CoV‐2 antibodies in COVID‐19 patients and healthy volunteers up to 6 months post disease onset

2020Journal article Open access
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dc.contributor.authorFigueiredo-Campos, Patricia
dc.contributor.authorBlankenhaus, Birte
dc.contributor.authorMota, Catarina
dc.contributor.authorGomes, Andreia
dc.contributor.authorSerrano, Marta
dc.contributor.authorAriotti, Silvia
dc.contributor.authorCosta, Catarina
dc.contributor.authorNunes-Cabaço, Helena
dc.contributor.authorMendes, António M.
dc.contributor.authorGaspar, Pedro
dc.contributor.authorPereira‐Santos, M. Conceição
dc.contributor.authorRodrigues, Fabiana
dc.contributor.authorCondeço, Jorge
dc.contributor.authorEscoval, M. Antonia
dc.contributor.authorSantos, Matilde
dc.contributor.authorRamirez, Mário
dc.contributor.authorCristino, José Melo
dc.contributor.authorSimas, J Pedro
dc.contributor.authorVasconcelos, Eugenia
dc.contributor.authorAfonso, Ângela
dc.contributor.authorVeldhoen, Marc
dc.date.accessioned2021-04-07T12:23:05Z
dc.date.available2021-04-07T12:23:05Z
dc.date.issued2020
dc.description© 2020 The Authors. European Journal of Immunology published by Wiley‐VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.pt_PT
dc.description.abstractSARS-CoV-2 has emerged as a human pathogen, causing clinical signs, from fever to pneumonia-COVID-19-but may remain mild or asymptomatic. To understand the continuing spread of the virus, to detect those who are and were infected, and to follow the immune response longitudinally, reliable and robust assays for SARS-CoV-2 detection and immunological monitoring are needed. We quantified IgM, IgG, and IgA antibodies recognizing the SARS-CoV-2 receptor-binding domain (RBD) or the Spike (S) protein over a period of 6 months following COVID-19 onset. We report the detailed setup to monitor the humoral immune response from over 300 COVID-19 hospital patients and healthcare workers, 2500 University staff, and 198 post-COVID-19 volunteers. Anti-SARS-CoV-2 antibody responses follow a classic pattern with a rapid increase within the first three weeks after symptoms. Although titres reduce subsequently, the ability to detect anti-SARS-CoV-2 IgG antibodies remained robust with confirmed neutralization activity for up to 6 months in a large proportion of previously virus-positive screened subjects. Our work provides detailed information for the assays used, facilitating further and longitudinal analysis of protective immunity to SARS-CoV-2. Importantly, it highlights a continued level of circulating neutralising antibodies in most people with confirmed SARS-CoV-2.pt_PT
dc.description.sponsorshipWe like to acknowledge the funding from the European Union H2020 ERA project (No 667824 – EXCELLtoINNOV) and the Fundação para a Ciência e a Tecnologia (FCT) to P.F‐C. (SFRH/BD/131605/2017), PTDC/MED‐IMU/28003/2017, and research4COVID19 (no. 231_596873172, Generating SARS‐CoV2 seroconversion assay and no. 729, High‐throughput SARS‐CoV2 neutralizing antibodies assessment), with additional support by Sociedade Francisco Manuel dos Santos.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEur J Immunol. 2020 Dec;50(12):2025-2040pt_PT
dc.identifier.doi10.1002/eji.202048970pt_PT
dc.identifier.eissn1521-4141
dc.identifier.issn0014-2980
dc.identifier.urihttp://hdl.handle.net/10451/47273
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJohn Wiley & Sons, Inc.pt_PT
dc.relationPTDC/MED‐IMU/28003/2017pt_PT
dc.relationLeverage research EXCELLence and INNOVation potential of Instituto de Medicina Molecular (IMM) through translational biomedical research in immunity and infection.
dc.relation“How to activate non-conventional Epithelial T cells”
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/journal/15214141pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCOVID-19pt_PT
dc.subjectSARS-CoV-2pt_PT
dc.subjectSeroprevalencept_PT
dc.subjectNeutralizing antibodiespt_PT
dc.titleSeroprevalence of anti‐SARS‐CoV‐2 antibodies in COVID‐19 patients and healthy volunteers up to 6 months post disease onsetpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleLeverage research EXCELLence and INNOVation potential of Instituto de Medicina Molecular (IMM) through translational biomedical research in immunity and infection.
oaire.awardTitle“How to activate non-conventional Epithelial T cells”
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/667824/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F131605%2F2017/PT
oaire.citation.endPage2040pt_PT
oaire.citation.issue12pt_PT
oaire.citation.startPage2025pt_PT
oaire.citation.titleEuropean Journal of Immunologypt_PT
oaire.citation.volume50pt_PT
oaire.fundingStreamH2020
oaire.fundingStreamOE
person.familyNameFigueiredo-Campos
person.familyNameBlankenhaus
person.familyNameMota da Silva
person.familyNameAriotti
person.familyNameNunes Cabaço
person.familyNameM. Mendes
person.familyNameGaspar
person.familyNameRamos de Almeida Ramirez
person.familyNameMelo Cristino
person.familyNameSimas
person.familyNameVeldhoen
person.givenNamePatricia
person.givenNameBirte
person.givenNameMaria Catarina
person.givenNameSilvia
person.givenNameHelena Isabel Marques
person.givenNameAntónio
person.givenNamePedro
person.givenNameMário Nuno
person.givenNameJosé
person.givenNameJ Pedro
person.givenNameMarc
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project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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