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Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study

2021-07-14Journal article Open access
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dc.contributor.authorCunha, Eva
dc.contributor.authorValente, Sara
dc.contributor.authorNascimento, Mariana
dc.contributor.authorPereira, Marcelo
dc.contributor.authorTavares, Luis
dc.contributor.authorDias, Ricardo
dc.contributor.authorOliveira, Manuela
dc.date.accessioned2021-11-04T22:31:56Z
dc.date.available2021-11-04T22:31:56Z
dc.date.issued2021-07-14
dc.descriptionResearch Areas: Science & Technology - Other Topicspt_PT
dc.description.abstractPeriodontal disease (PD) is one of the most widespread inflammatory diseases in dogs. This disease is initiated by a polymicrobial biofilm in the teeth surface (dental plaque), leading to a local inflammatory response, with gingivitis and/or several degrees of periodontitis. For instance, the prevention of bacterial dental plaque formation and its removal are essential steps in PD control. Recent research revealed that the antimicrobial peptide nisin incorporated in the delivery system guar gum (biogel) can inhibit and eradicate bacteria from canine dental plaque, being a promising compound for prevention of PD onset in dogs. However, no information is available regarding its effect on the dog's oral microbiome. In this pilot study, the influence of the nisin-biogel on the diversity of canine oral microbiome was evaluated using next generation sequencing (NGS), aiming to access the viability of nisin-biogel to be used in long-term experiment in dogs. Composite toothbrushing samples of the supragingival plaque from two dogs were collected at three timepoints: T1-before any application of the nisin-biogel to the animals' teeth surface; T2-one hour after one application of the nisin-biogel; and T3-one hour after a total of three applications of the nisin-biogel, each 48 hours. After that, microbial profiling was performed by NGS of the V3V4 16s rRNA region. After only one application of the nisin-biogel to the oral cavity of dogs, a statistically significant reduction in microbial diversity was observed (T2) as well as a reduction of some bacterial species potentially related with distinct stages of PD, when compared with samples collected before any application (T1). However, after a total of three nisin-biogel applications (T3), a recovery of the microbial diversity was detected. In conclusion, the nisin-biogel may influence the canine oral microbiome. A reduction in some bacterial species potentially related with distinct stages of PD was observed. This pilot study will help to design a controlled in vivo clinical trial to evaluate nisin-biogel effect on dental plaque progression and canine periodontal indices evolution in a long-term application period.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCunha E, Valente S, Nascimento M, Pereira M, Tavares L, Dias R, Oliveira M. 2021. Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study. PeerJ 9:e11626. DOI:10.7717/peerj.11626pt_PT
dc.identifier.doi10.7717/peerj.11626pt_PT
dc.identifier.issn2167-8359
dc.identifier.urihttp://hdl.handle.net/10400.5/22505
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPEERJ INCpt_PT
dc.relation.publisherversionhttps://peerj.com/articles/11626/#pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectNisin-biogelpt_PT
dc.subjectOral microbiomept_PT
dc.subjectPeriodontal diseasept_PT
dc.subjectDogspt_PT
dc.titleInfluence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot studypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157379/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/68219/PT
oaire.citation.conferencePlaceEnglandpt_PT
oaire.citation.issuee11626pt_PT
oaire.citation.titlePeerJpt_PT
oaire.citation.volume9pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
person.familyNameGonçalves da Cunha
person.familyNameNascimento
person.familyNameMonteiro Pereira
person.familyNameMorgado Tavares
person.familyNameDias
person.familyNameOliveira
person.givenNameEva Sofia
person.givenNameMariana Mara
person.givenNameMarcelo Luís
person.givenNameLuis Manuel
person.givenNameRicardo
person.givenNameManuela
person.identifier.ciencia-id9811-7B6B-E2B9
person.identifier.ciencia-id4417-19C0-D3D2
person.identifier.ciencia-id9014-140F-3022
person.identifier.ciencia-id9011-18C6-482D
person.identifier.ciencia-idBE1E-2B3B-92D2
person.identifier.orcid0000-0002-5641-4400
person.identifier.orcid0000-0002-1424-9275
person.identifier.orcid0000-0001-8671-6285
person.identifier.orcid0000-0002-6887-2361
person.identifier.orcid0000-0002-3910-1525
person.identifier.scopus-author-id7102885098
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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