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Validation of a quantitative cerebrospinal fluid alpha-synuclein assay in a European-wide interlaboratory study

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dc.contributor.authorKruse, Niels
dc.contributor.authorPersson, Staffan
dc.contributor.authorAlcolea, Daniel
dc.contributor.authorBahl, Justyna M.C.
dc.contributor.authorBaldeiras, Ines
dc.contributor.authorCapello, Elisabetta
dc.contributor.authorChiasserini, Davide
dc.contributor.authorBocchio Chiavetto, Luisella
dc.contributor.authorEmersic, Andreja
dc.contributor.authorEngelborghs, Sebastiaan
dc.contributor.authorEren, Erden
dc.contributor.authorFladby, Tormod
dc.contributor.authorFrisoni, Giovanni
dc.contributor.authorGarcía-Ayllón, María-Salud
dc.contributor.authorGenc, Sermin
dc.contributor.authorGkatzima, Olymbia
dc.contributor.authorHeegaard, Niels H.H.
dc.contributor.authorJaneiro, André M.
dc.contributor.authorKováčech, Branislav
dc.contributor.authorKuiperij, H. Bea
dc.contributor.authorLeitão, Maria J.
dc.contributor.authorLleó, Alberto
dc.contributor.authorMartins, Madalena
dc.contributor.authorRamos De Matos, Mafalda
dc.contributor.authorMollergard, Hanne M.
dc.contributor.authorNobili, Flavio
dc.contributor.authorÖhrfelt, Annika
dc.contributor.authorParnetti, Lucilla
dc.contributor.authorde Oliveira, Catarina Resende
dc.contributor.authorRot, Uros
dc.contributor.authorSáez-Valero, Javier
dc.contributor.authorStruyfs, Hanne
dc.contributor.authorTanassi, Julia T.
dc.contributor.authorTaylor, Peggy
dc.contributor.authorTsolaki, Magda
dc.contributor.authorVanmechelen, Eugeen
dc.contributor.authorVerbeek, Marcel M.
dc.contributor.authorZilka, Norbert
dc.contributor.authorBlennow, Kaj
dc.contributor.authorZetterberg, Henrik
dc.contributor.authorMollenhauer, Brit
dc.date.accessioned2022-05-30T15:39:22Z
dc.date.available2022-05-30T15:39:22Z
dc.date.issued2015
dc.description© 2015 Elsevier Inc. All rights reserved.pt_PT
dc.description.abstractDecreased levels of alpha-synuclein (aSyn) in cerebrospinal fluid (CSF) in Parkinson's disease and related synucleinopathies have been reported, however, not consistently in all cross-sectional studies. To test the performance of one recently released human-specific enzyme-linked immunosorbent assay (ELISA) for the quantification of aSyn in CSF, we carried out a round robin trial with 18 participating laboratories trained in CSF ELISA analyses within the BIOMARKAPD project in the EU Joint Program - Neurodegenerative Disease Research. CSF samples (homogeneous aliquots from pools) and ELISA kits (one lot) were provided centrally and data reported back to one laboratory for data analysis. Our study showed that although factors such as preanalytical sample handling and lot-to-lot variability were minimized by our study design, we identified high variation in absolute values of CSF aSyn even when the same samples and same lots of assays were applied. We further demonstrate that although absolute concentrations differ between laboratories the quantitative results are comparable. With further standardization this assay may become an attractive tool for comparing aSyn measurements in diverse settings. Recommendations for further validation experiments and improvement of the interlaboratory results obtained are given.pt_PT
dc.description.sponsorshipThis is an EU Joint Program – Neurodegenerative Disease Research (JPND) project. The project is supported through the following funding organizations under the aegis of JPND–www.jpnd.eu: The Alzheimer Research Foundation (SAO-FRA), Belgium (grant number S#12018); Danish Council for Strategic Research, Denmark (grant number 0603-00511B); Bundesministerium für Bildung und Forschung (BMBF), Germany (grant number OIED1203C); General Secretary of Research and Technology, Greece; Italian Ministry of Health (Ricerca Corrente), Italy; The Fundação para a Ciência e a Tecnologia, Portugal (grant numbers JPND/0004/2011, IMM/BPD/51-2013, IMM/BI/7-2013, JPND/0005/2011); Fondo de Investigaciones Sanitarias, Instituto de Salud Carlos III, Spain (grant numbers PI11/03026, PI11/03035; cofinanced by the Fondo Europeo de Desarrollo Regional); The Ministry of Education, Research and Sport of the Slovak Republic, Slovakia; The Slovenian Research Agency, Slovenia (grant number A3-0001); The Swedish Research Council, Sweden; Ministry of Education for Netherlands: ZonMw (The Netherlands Organisation for Health Research and Development), The Netherlands (grant number 629000002); The Scientific and Technological Research Council of Turkey (grant number 112S335).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNeurobiol Aging . 2015 Sep;36(9):2587-2596pt_PT
dc.identifier.doi10.1016/j.neurobiolaging.2015.05.003pt_PT
dc.identifier.eissn1558-1497
dc.identifier.issn0197-4580
dc.identifier.urihttp://hdl.handle.net/10451/53224
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationIMM/BPD/51-2013pt_PT
dc.relationIMM/BI/7-2013pt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/neurobiology-of-agingpt_PT
dc.subjectAlpha-synucleinpt_PT
dc.subjectBiomarkerpt_PT
dc.subjectCerebrospinal fluidpt_PT
dc.subjectELISApt_PT
dc.subjectValidationpt_PT
dc.titleValidation of a quantitative cerebrospinal fluid alpha-synuclein assay in a European-wide interlaboratory studypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberJPND/0004/2011
oaire.awardNumberJPND/0005/2011
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/JPND%2F0004%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/JPND%2F0005%2F2011/PT
oaire.citation.endPage2596pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage2587pt_PT
oaire.citation.titleNeurobiology of Agingpt_PT
oaire.citation.volume36pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
person.familyNameda Rocha Martins
person.familyNameRamos de Matos
person.givenNameMadalena Cristina
person.givenNameMafalda
person.identifier.ciencia-id3913-56BA-8AD6
person.identifier.orcid0000-0001-8360-6806
person.identifier.orcid0000-0002-4206-2854
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationd256ee78-61aa-4745-b73a-9763278e6e0c
relation.isAuthorOfPublication712894e0-c03d-4290-b28a-730c933694f1
relation.isAuthorOfPublication.latestForDiscoveryd256ee78-61aa-4745-b73a-9763278e6e0c
relation.isProjectOfPublication157ae5bb-2898-4820-a854-8862e7cbb657
relation.isProjectOfPublication30fc1b73-3eb2-4e82-8607-66c6aa5180df
relation.isProjectOfPublication.latestForDiscovery30fc1b73-3eb2-4e82-8607-66c6aa5180df

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