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Eficácia antimicrobiana de medicação intracanalar de nanopartículas MgO-400
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Objetivo: Comparar a eficácia antimicrobiana de medicação intracanalar constituída por nanopartículas de óxido de magnésio (MgO) com pasta de hidróxido de cálcio (HC), pasta bi-antibiótica (PBA), e com o veículo de administração incorporado nas pastas, o polietilenoglicol (PEG), face à Enterococcus faecalis.
Métodos: Uma amostra constituída por 54 dentes foi preparada, esterilizada e dividida nos seguintes grupos (n=9): (A) – pasta nano-MgO; (B) – HC comercial; (C) – PBA (ciprofloxacina e metronidazol); (D) – PEG; (-) – controlo negativo; (+) – controlo positivo. A avaliação preliminar in vitro do potencial antimicrobiano foi realizada através do teste spot-on-lawn e difusão em poço. Adicionalmente, determinou-se a concentração mínima inibitória (CMI) e bactericida (CMB) das nano-MgO. Na fase ex vivo, os dentes foram inoculados com a estirpe de referência E. faecalis ATCC 51299 e incubados a 37ºC por 24 h, ao fim das quais foram colocados os compostos. Após sete dias de incubação as pastas foram removidas e a multiplicação bacteriana foi avaliada às 24, 48 e 72 h pela observação de turvação do meio de cultura e medição da densidade ótica às 24 h. Os resultados foram avaliados através do teste exato de Fisher, sendo p <0,05 considerado como estatisticamente significativo.
Resultados: Nos testes in vitro verificou-se que a PBA apresentou o maior halo de inibição. As nano-MgO apresentaram uma CMI de 0,78 mg/mL e uma CMB superior a 2,5 mg/mL. Na análise ex vivo, a PBA apresentou diferença estatisticamente significativa em relação aos restantes compostos e uma percentagem de inibição bacteriana superior (98,33%).
Conclusões: Nas condições deste estudo constatou-se que as nanopartículas de MgO apresentam um potencial antimicrobiano inferior à PBA, tanto in vitro como ex vivo. Sugerem-se estudos no âmbito clínico com nano-MgO, considerando o revestimento com PEG e análise da microestrutura das nanopartículas, bem como a sua aplicação noutras fases do tratamento endodôntico.
Aim: To compare the antimicrobial activity of an intracanal paste composed by magnesium oxide (MgO) nanoparticles with calcium hydroxide (CH), a bi-antibiotic paste (BAP) and polyethylene glycol (PEG) against E. faecalis. Methods: A sample of 54 teeth was prepared, sterilized, and divided into the following groups (n=9): (A) – nano-MgO paste; (B) – commercial CH; (C) – BAP (ciprofloxacin and metronidazole); (D) – PEG; (-) – negative control; (+) – positive control. The preliminary in vitro evaluation of the antimicrobial potential was performed through a spot-on-lawn and well diffusion test. Additionally, the minimum inhibitory (MIC) and bactericidal (MBC) concentrations for the MgO nanoparticles were determined. For the ex vivo experiment, teeth were inoculated with the reference strain E. faecalis ATCC 51299 and incubated at 37°C for 24 hours. After that, the respective compounds were applied. The pastes were removed after seven days of incubation, and their antimicrobial activity was evaluated at 24, 48 and 72 h, by observing the teeth and paper points tubes’ turbidity and through the optical density at 24 h. The data were analysed using Fisher's exact test, and a p-value <0.05 was considered statistically significant. Results: In vitro tests showed that BAP presented a bigger inhibition zone halo. The MgO nanoparticle solution presented a MIC of 0.78 mg/mL and a MBC superior to 2.5 mg/mL. In the ex vivo evaluation, BAP showed statistically significant results, comparing with the other compounds, besides a higher percentage of bacterial inhibition (98.33%). Conclusion: In the conditions of this study, it was found that the antimicrobial activity of the MgO nanoparticle paste fell below the expectations, and the BAP exhibited superior antimicrobial activity compared to the other pastes. Clinical studies are suggested, also the coating of the nano-MgO with PEG and the microstructure analysis should be performed, these studies may be focused on other treatment phases.
Aim: To compare the antimicrobial activity of an intracanal paste composed by magnesium oxide (MgO) nanoparticles with calcium hydroxide (CH), a bi-antibiotic paste (BAP) and polyethylene glycol (PEG) against E. faecalis. Methods: A sample of 54 teeth was prepared, sterilized, and divided into the following groups (n=9): (A) – nano-MgO paste; (B) – commercial CH; (C) – BAP (ciprofloxacin and metronidazole); (D) – PEG; (-) – negative control; (+) – positive control. The preliminary in vitro evaluation of the antimicrobial potential was performed through a spot-on-lawn and well diffusion test. Additionally, the minimum inhibitory (MIC) and bactericidal (MBC) concentrations for the MgO nanoparticles were determined. For the ex vivo experiment, teeth were inoculated with the reference strain E. faecalis ATCC 51299 and incubated at 37°C for 24 hours. After that, the respective compounds were applied. The pastes were removed after seven days of incubation, and their antimicrobial activity was evaluated at 24, 48 and 72 h, by observing the teeth and paper points tubes’ turbidity and through the optical density at 24 h. The data were analysed using Fisher's exact test, and a p-value <0.05 was considered statistically significant. Results: In vitro tests showed that BAP presented a bigger inhibition zone halo. The MgO nanoparticle solution presented a MIC of 0.78 mg/mL and a MBC superior to 2.5 mg/mL. In the ex vivo evaluation, BAP showed statistically significant results, comparing with the other compounds, besides a higher percentage of bacterial inhibition (98.33%). Conclusion: In the conditions of this study, it was found that the antimicrobial activity of the MgO nanoparticle paste fell below the expectations, and the BAP exhibited superior antimicrobial activity compared to the other pastes. Clinical studies are suggested, also the coating of the nano-MgO with PEG and the microstructure analysis should be performed, these studies may be focused on other treatment phases.
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Teses de mestrado - 2023 Saúde Oral