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Assessment of the presence of Hepatitis E virus in surface water and drinking water in Portugal

2020Journal article Open access
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dc.contributor.authorSalvador, Daniel
dc.contributor.authorNeto, Célia
dc.contributor.authorBenoliel, Maria João
dc.contributor.authorCaeiro, M. F.
dc.date.accessioned2020-05-27T13:28:08Z
dc.date.available2020-05-27T13:28:08Z
dc.date.issued2020
dc.description© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).pt_PT
dc.description.abstractHepatitis E virus (HEV) is a non-enveloped single-stranded positive-sense RNA virus, belonging to the Hepeviridae family, resistant to environmental conditions, and transmitted by the consumption of contaminated water. This virus is responsible for both sporadic and epidemic outbreaks, leading to thousands of infections per year in several countries, and is thus considered an emerging disease in Europe and Asia. This study refers to a survey in Portugal during 2019, targeting the detection and eventual quantification of enteric viruses in samples from surface and drinking water. Samples positive for HEV RNA were recurrently found by reverse transcription quantitative PCR (RT-qPCR), in both types of matrix. The infectivity of these samples was evaluated in cultured Vero E6 cells and RNA from putative viruses produced in cultures evidencing cytopathic effects and was subjected to RT-qPCR targeting HEV genomic RNA. Our results evidenced the existence of samples positive either for HEV RNA (77.8% in surface water and 66.7% in drinking water) or for infectious HEV (23.0% in surface water and 27.7% in drinking water). These results highlight the need for effective virological control of water for human consumption and activities.pt_PT
dc.description.sponsorshipThis research was funded by Empresa Portuguesa das Águas Livres (EPAL) and Foundation for Science and Technology (FCT) Portugal through a Ph.D. grant to D. Salvador (PDE/BDE/114582/2016) and FCT/MCTES projects UIDP/50017/2020+UIDB/50017/2020 and UIDB/04295/2020 through national funds.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicroorganisms 2020, 8(5), 761pt_PT
dc.identifier.doi10.3390/microorganisms8050761pt_PT
dc.identifier.eissn2076-2607
dc.identifier.urihttp://hdl.handle.net/10451/43723
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationAs cianotoxinas nas àguas de consumo e a incidência de cancro em Portugal -Deteção e quantificação de enterovirus, vírus das hepatites A e E em águas de consumo e residuais
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.relationEnvironmental Health Institute
dc.relation.publisherversionhttps://www.mdpi.com/journal/microorganismspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDrinking waterpt_PT
dc.subjectEnteric virusespt_PT
dc.subjectHepatitis E viruspt_PT
dc.subjectRT-qPCRpt_PT
dc.subjectSurface waterpt_PT
dc.subjectVero E6 cell linept_PT
dc.subjectViral infectivitypt_PT
dc.subjectWater qualitypt_PT
dc.subjectWater treatmentpt_PT
dc.titleAssessment of the presence of Hepatitis E virus in surface water and drinking water in Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAs cianotoxinas nas àguas de consumo e a incidência de cancro em Portugal -Deteção e quantificação de enterovirus, vírus das hepatites A e E em águas de consumo e residuais
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleEnvironmental Health Institute
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/PDE%2FBDE%2F114582%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04295%2F2020/PT
oaire.citation.issue5pt_PT
oaire.citation.startPage761pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume8pt_PT
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSalvador
person.familyNameCaeiro
person.givenNameDaniel
person.givenNameMaria
person.identifier346320
person.identifier.ciencia-idBC19-2AD3-CFEE
person.identifier.ciencia-id0415-02D2-70C5
person.identifier.orcid0000-0003-2278-9877
person.identifier.orcid0000-0003-0559-6344
person.identifier.ridC-1200-2014
person.identifier.scopus-author-id57110214500
person.identifier.scopus-author-id8560687400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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