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Epidemic history of hepatitis C virus genotypes and subtypes in Portugal

2018-12Journal article Open access
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dc.contributor.authorPalladino, Claudia
dc.contributor.authorEzeonwumelu, Ifeanyi J.
dc.contributor.authorMarcelino, Rute
dc.contributor.authorBriz, Verónica
dc.contributor.authorMoranguinho, Inês
dc.contributor.authorSerejo, Fátima
dc.contributor.authorVelosa, José Fernando
dc.contributor.authorMarinho, Rui
dc.contributor.authorBorrego, Pedro
dc.contributor.authorTaveira, Nuno
dc.date.accessioned2023-08-31T14:59:42Z
dc.date.available2023-08-31T14:59:42Z
dc.date.issued2018-12
dc.date.updated2023-02-09T14:44:33Z
dc.description.abstractAny successful strategy to prevent and control HCV infection requires an understanding of the epidemic behaviour among the different genotypes. Here, we performed the first characterization of the epidemic history and transmission dynamics of HCV subtypes in Portugal. Direct sequencing of NS5B was performed on 230 direct-acting antiviral drugs (DAA)-treatment naïve patients in Lisbon. Phylogenetic analysis was used for subtyping and transmission cluster identification. Bayesian methods were used to reconstruct the epidemic history of HCV subtypes. Sequences were analysed for resistance-associated substitutions (RAS). The majority of strains were HCV-GT1 (62.6%), GT3 (18.3%, all subtype 3a) and GT4 (16.1%). Among GT1, the most frequent were subtypes 1a (75.5%) and 1b (24.5%). Polyphyletic patterns were found in all but 12 lineages suggesting multiple introductions of the different subtypes in this population. Five distinct epidemics were identified. The first significant HCV epidemic in Portugal occurred between 1930s and 1960s, was caused almost exclusively by GT1b and was likely associated with blood transfusions. Rapid expansion of GT3a occurred in the 1960s and GT1a in the 1980s, associated with intravenous drug use. The most recent epidemics were caused by GT4a and GT4d and seem to be associated with the resurgence of opioid use. The C316N substitution was found in 31.4% of GT1b-patients. Close surveillance of patients bearing this mutation and undergoing dasabuvir-based regimens will be important to determine its impact on treatment outcome.pt_PT
dc.description.sponsorshipThe authors would like to thank all the patients for their participation. C.P. is supported by the Portuguese ‘Fundação para a Ciência e Tecnologia’ (FCT) (grant number SFRH/BPD/77448/2011, part of the EDCTP2 program supported by the European Union). R.M. was supported by a Ph.D. scholarship by the Portuguese ‘Fundação para a Ciência e Tecnologia’ (FCT). V.B. is supported by the Miguel Servet program run by the ‘Fondo de Investigación Sanitaria’ (ISCIII) (grant number CP13/00098). I.M. is supported by the Portuguese ‘Fundação para a Ciência e Tecnologia’ (FCT) (grant number SFRH/BD/131062/2017).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPalladino C, Ezeonwumelu IJ, Marcelino R, Briz V, Moranguinho I, Serejo F, et al. Epidemic history of hepatitis C virus genotypes and subtypes in Portugal. Sci Rep [Internet]. 16 de agosto de 2018;8(1):12266. Disponível em: https://www.nature.com/articles/s41598-018-30528-0pt_PT
dc.identifier.doi10.1038/s41598-018-30528-0pt_PT
dc.identifier.slugcv-prod-1185522
dc.identifier.urihttp://hdl.handle.net/10451/59110
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.relationEPIDEMIOLOGY, PATHOGENESIS AND DETERMINANTS OF HIV-1 TRANSMISSION AND DISEASE PROGRESSION IN THE ANGOLAN PERINATAL HIV COHORT APEHC
dc.relationGene therapy for HIV cure using RNA replicons.
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-018-30528-0pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleEpidemic history of hepatitis C virus genotypes and subtypes in Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEPIDEMIOLOGY, PATHOGENESIS AND DETERMINANTS OF HIV-1 TRANSMISSION AND DISEASE PROGRESSION IN THE ANGOLAN PERINATAL HIV COHORT APEHC
oaire.awardTitleGene therapy for HIV cure using RNA replicons.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBPD%2F77448%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F131062%2F2017/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage12266pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume8pt_PT
oaire.fundingStreamOE
person.familyNamePalladino
person.familyNameEzeonwumelu
person.familyNameCarvalho Antunes Marcelino
person.familyNameBriz
person.familyNameMoranguinho
person.familyNameMarinho
person.familyNameBorrego
person.familyNameTaveira
person.givenNameClaudia
person.givenNameIfeanyi
person.givenNameRute Alexandra
person.givenNameVerónica
person.givenNameInês
person.givenNameRui
person.givenNamePedro
person.givenNameNuno
person.identifier1927668
person.identifier.ciencia-id721D-6851-20D5
person.identifier.ciencia-idA417-7162-733D
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person.identifier.ciencia-id531A-BB6A-AA45
person.identifier.ciencia-idA71C-0177-722F
person.identifier.ciencia-id7111-3383-A926
person.identifier.orcid0000-0002-8148-0928
person.identifier.orcid0000-0003-4007-2212
person.identifier.orcid0000-0002-4535-9725
person.identifier.orcid0000-0003-2297-5098
person.identifier.orcid0000-0003-1327-3537
person.identifier.orcid0000-0002-1949-9484
person.identifier.orcid0000-0003-0176-5585
person.identifier.ridP-9428-2017
person.identifier.ridT-4183-2017
person.identifier.ridH-6968-2013
person.identifier.scopus-author-id57203432749
person.identifier.scopus-author-id9332849600
person.identifier.scopus-author-id7005027284
person.identifier.scopus-author-id25225039400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid721D-6851-20D5 | Claudia Palladino
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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