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Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi

2022Artigo científico Acesso aberto
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dc.contributor.authorPlácido, Alexandra
dc.contributor.authorAmaral, Constança Pais Do
dc.contributor.authorTeixeira, Cátia
dc.contributor.authorNogueira, Ariane
dc.contributor.authorBrango‐Vanegas, José
dc.contributor.authorAlves Barbosa, Eder
dc.contributor.authorC. Moreira, Daniel
dc.contributor.authorSilva‐Carvalho, Amandda E.
dc.contributor.authorda Silva, Maria da Gloria
dc.contributor.authordo Nascimento Dias, Jhones
dc.contributor.authorAlbuquerque, Patrícia
dc.contributor.authorSaldanha‐Araújo, Felipe
dc.contributor.authorC. D. A. Lima, Filipe
dc.contributor.authorBatagin‐Neto, Augusto
dc.contributor.authorKuckelhaus, Selma
dc.contributor.authorBessa, Lucinda J.
dc.contributor.authorFreitas, Jaime
dc.contributor.authorDotto Brand, Guilherme
dc.contributor.authorSantos, Nuno C.
dc.contributor.authorB. Relvas, João
dc.contributor.authorGomes, Paula
dc.contributor.authorS. A. Leite, José Roberto
dc.contributor.authorEaton, Peter
dc.date.accessioned2022-04-26T16:25:14Z
dc.date.available2022-04-26T16:25:14Z
dc.date.issued2022
dc.description© 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly citedpt_PT
dc.description.abstractTryptophyllins constitute a heterogeneous group of peptides that are one of the first classes of peptides identified from amphibian's skin secretions. Here, we report the structural characterization and antioxidant properties of a novel tryptophyllin-like peptide, named PpT-2, isolated from the Iberian green frog Pelophylax perezi. The skin secretion of P. perezi was obtained by electrical stimulation and fractionated using RP-HPLC. De novo peptide sequencing was conducted using MALDI MS/MS. The primary structure of PpT-2 (FPWLLS-NH2 ) was confirmed by Edman degradation and subsequently investigated using in silico tools. PpT-2 shared physicochemical properties with other well-known antioxidants. To test PpT-2 for antioxidant activity in vitro, the peptide was synthesized by solid phase and assessed in the chemical-based ABTS and DPPH scavenging assays. Then, a flow cytometry experiment was conducted to assess PpT-2 antioxidant activity in oxidatively challenged murine microglial cells. As predicted by the in silico analyses, PpT-2 scavenged free radicals in vitro and suppressed the generation of reactive species in PMA-stimulated BV-2 microglia cells. We further explored possible bioactivities of PpT-2 against prostate cancer cells and bacteria, against which the peptide exerted a moderate antiproliferative effect and negligible antimicrobial activity. The biocompatibility of PpT-2 was evaluated in cytotoxicity assays and in vivo toxicity with Galleria mellonella. No toxicity was detected in cells treated with up to 512 µg/ml and in G. mellonella treated with up to 40 mg/kg PpT-2. This novel peptide, PpT-2, stands as a promising peptide with potential therapeutic and biotechnological applications, mainly for the treatment/prevention of neurodegenerative disorders.pt_PT
dc.description.sponsorshipThis work was financed by FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 - Operacional Programme for Competitiveness and Internationalization (POCI), and by Portuguese funds through FCT - Fundação para a Ciência e a Tecnologia in the framework of the project POCI-01-0145-FEDER-031158 – PTDC/BII-BIO/31158/2017. The authors would like to thank the participation and scientific support of the Unit projects UIDB/50006/2020 | UIDP/50006/2020, and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Universal Faixa ‘B’ (grant number 32103/2018-0). A.P. is a recipient of a post-doctoral grant from the project PTDC/BII-BIO/31158/2017. The authors would like to thank the researcher Roberto Resendes (CiBio, University of the Azores, Ponta Delgada, São Miguel, Azores, Portugal) for the logistical support in the collection of samples. C.P.A acknowledges FCT-MCTES fellowship PD/BD/136860/2018. A.B.-N. and F.C.D.A.L. acknowledge CNPq (grants 420449/2018-3 and 428211/2018-6) for financial support.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Cell Mol Med. 2022 Apr 23pt_PT
dc.identifier.doi10.1111/jcmm.17292pt_PT
dc.identifier.eissn1582-4934
dc.identifier.issn1582-1838
dc.identifier.urihttp://hdl.handle.net/10451/52554
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationPOCI-01-0145-FEDER-031158pt_PT
dc.relationValorisation of Molecules Isolated from Aquatic Portuguese Amphibians
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationCharacterization and evaluation of therapeutic potential of anticancer and antibacterial peptides isolated from reptiles.
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/journal/15824934pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPelophylax perezipt_PT
dc.subjectAmphibiapt_PT
dc.subjectAntioxidantpt_PT
dc.subjectBioactive peptidept_PT
dc.subjectNeuroprotectionpt_PT
dc.subjectTryptophyllinpt_PT
dc.titleNeuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleValorisation of Molecules Isolated from Aquatic Portuguese Amphibians
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleCharacterization and evaluation of therapeutic potential of anticancer and antibacterial peptides isolated from reptiles.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBII-BIO%2F31158%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PD%2FBD%2F136860%2F2018/PT
oaire.citation.titleJournal of Cellular and Molecular Medicinept_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePais do Amaral
person.familyNameTeixeira
person.familyNameSantos
person.givenNameConstança
person.givenNameCátia
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person.identifier.ridN-7248-2013
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project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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