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A non-invasive method based on saliva to characterize transthyretin in familial amyloidotic polyneuropathy patients using FT-ICR high-resolution MS

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dc.contributor.authorCosta, Gonçalo da
dc.contributor.authorGuerreiro, Ana
dc.contributor.authorCorreia, Catarina F.
dc.contributor.authorGomes, Ricardo J.
dc.contributor.authorFreire, António
dc.contributor.authorMonteiro, Estela
dc.contributor.authorBarroso, Eduardo
dc.contributor.authorCoelho, Ana V.
dc.contributor.authorOuteiro, Tiago F.
dc.contributor.authorFreire, Ana Ponces
dc.contributor.authorCordeiro, Carlos
dc.date.accessioned2012-05-25T15:34:48Z
dc.date.available2012-05-25T15:34:48Z
dc.date.issued2010
dc.descriptionCopyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheimpor
dc.descriptionDefinitive version in http://www.interscience.wiley.com/eng
dc.description.abstractPurpose: To identify, characterize and perform a relative quantification of human transthyretin (TTR) variants in human saliva. Experimental design: Serum and saliva samples were collected from healthy and familial amyloidotic polyneuropathy (FAP) patients, proteins separated by SDS-PAGE, TTR bands excised, in-gel digested and analyzed by MALDI-FTICR. Results: We identified and performed a relative quantification of mutated and native TTR forms in human saliva, based on FTICR-MS. The results are quantitatively identical to the ones obtained with human serum. In FAP patients subjected to cadaveric liver transplant, the TTR mutant form is no longer detected in saliva, while in patients receiving a domino liver from a FAP donor the mutant form of TTR becomes detectable in saliva, thus demonstrating the serum origin of TTR in saliva. Conclusions and clinical relevance: Saliva TTR originates in serum and the ratio of mutant to native TTR is preserved. The method provides a non-invasive detection of mutated TTR and a relative quantification of TTR forms. Diagnostic and disease prognosis of FAP is crucial at early stages of the disease and after liver transplantation, the only curative therapy. A suitable noninvasive method was developed for monitoring the most important FAP biomarker in human salivaeng
dc.description.sponsorshipWe also acknowledge the Fundação para a Ciência e a Tecnologia do Ministério da Ciência Tecnologia e Ensino Superior, Portugal, for the Intrument Network Grant REDE/1501/REM/2005 and for grant PDTC/QUI/70610/2006eng
dc.identifier.citationProteomics Clin. Appl. 2010, 4, 674–678por
dc.identifier.issn1862-8346
dc.identifier.urihttp://hdl.handle.net/10451/6376
dc.identifier.urihttp://onlinelibrary.wiley.com/doi/10.1002/prca.200900118/pdf
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.subjectFamilial amyloidotic polyneuropathyeng
dc.subjectFTICReng
dc.subjectSalivaeng
dc.subjectSystemic amyloidosiseng
dc.subjectTransthyretineng
dc.titleA non-invasive method based on saliva to characterize transthyretin in familial amyloidotic polyneuropathy patients using FT-ICR high-resolution MSeng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage678por
oaire.citation.startPage674por
oaire.citation.titlePROTEOMICS - Clinical Applicationspor
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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