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Characterization of the transcriptional signatures associated with resistance and susceptibility to Hemileia vastatrix in the Kawisari coffee hybrid

2023Conference object Open access
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dc.contributor.authorAzevedo, Herlander
dc.contributor.authorDiniz, Inês
dc.contributor.authorFigueiredo, Andreia
dc.contributor.authorGuedes, Fernanda Alves Freitas
dc.contributor.authorSebastiana, Mónica Guita
dc.contributor.authorAzinheira, Helena Gil
dc.contributor.authorBarros, Danielle
dc.contributor.authorCastro, Pedro Humberto
dc.contributor.authorFreitas, Sara
dc.contributor.authorLoureiro, Andreia
dc.contributor.authorMuñoz-Pajares, Antonio Jesus
dc.contributor.authorPereira, Ana Paula
dc.contributor.authorSottomayor, Mariana
dc.contributor.authorTavares, Sílvia
dc.contributor.authorVarzea, Vitor
dc.contributor.authorGuerra-Guimarães, Leonor
dc.contributor.authorBatista, Dora
dc.contributor.authorSilva, Maria do Céu
dc.date.accessioned2023-07-21T11:41:47Z
dc.date.available2023-07-21T11:41:47Z
dc.date.issued2023
dc.description.abstractCoffee leaf rust (CLR), a disease caused by the biotrophic fungus Hemileia vastatrix (Hv), is the main threat to the worldwide production of Arabica coffee. The gradual breakdown of resistance in coffee varieties in the last years has highlighted the need for novel sources of resistance to CLR. This work aimed to unveil the cellular and molecular resistance profile of the Kawisari hybrid (C. arabica x C. liberica), a genotype used as a resistance donor in Arabica breeding programs in India. This coffee genotype was inoculated with two Hv races that triggered either resistance or susceptibility. Progress of infection was monitored using light microscopy. Simultaneously, we conducted a time-course RNA-seq characterization of the transcriptional responses. The microscopic studies showed that the post-haustorial resistance of Kawisari was associated with the hypersensitive response, accumulation of phenolic-like compounds and haustorium encasement with callose. The transcriptomic analysis suggest the downregulation of host primary metabolism genes at the early onset of infection, followed later by activation of genes functionally associated with multiple plant defense responses, including salicylic acid and jasmonate hormonal signaling. Resistance was also accompanied by the differential regulation of genes associated with phenylpropanoid metabolism and lignin biosynthesis. Our results, further validated by qPCR, provide important new insight into the molecular mechanisms underpinning resistance against CLR in this coffee genotype.pt_PT
dc.description.sponsorshipFoundation for Science and Technology (FCT) and FEDER funds through PORNorte under the project CoffeeRES PTDC/ASPPLA/ 29779/2017 and by FCT UNIT LEAF (UID/AGR/04129/2020).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAzevedo H, Diniz I, Figueiredo A, Guedes FAF, Sebastiana MG, Azinheira HG, Barros D, Castro, PH, Loureiro A, Muñoz-Pajares AJ, Pereira AP, Sottomayor M, Tavares S, Várzea V, Guerra-Guimarães, Batista D, Silva MC. (2023). Characterization of the transcriptional signatures associated with resistance and susceptibility to Hemileia vastatrix in the Kawisari coffee hybrid. Translational Research in Crops. Abstract. 22-23 June 2023, Ghent, Belgium. (Poster)pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/28027
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationPTDC/ASPPLA/29779/2017pt_PT
dc.relationUID/AGR/04129/2020pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCoffeept_PT
dc.subjectCoffee leaf rustpt_PT
dc.subjectHemileia vastatrixpt_PT
dc.subjectArabica coffeept_PT
dc.titleCharacterization of the transcriptional signatures associated with resistance and susceptibility to Hemileia vastatrix in the Kawisari coffee hybridpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlace22-23 June 2023, Ghent, Belgium.pt_PT
oaire.citation.titleTranslational Research in Cropspt_PT
person.familyNameDiniz
person.familyNameGil Azinheira
person.familyNameBrotas da Costa Loureiro
person.familyNameTavares
person.familyNameVarzea
person.familyNameGuerra-Guimarães
person.familyNameBatista
person.familyNameSilva
person.givenNameInês
person.givenNameHelena Manuela
person.givenNameAndreia Sofia
person.givenNameSílvia
person.givenNameVitor
person.givenNameLeonor
person.givenNameDora
person.givenNameMaria do Céu
person.identifierI-8326-2017
person.identifierG-4508-2014
person.identifier186839
person.identifierN-7385-2013
person.identifier.ciencia-idC317-320A-0AC0
person.identifier.ciencia-idD715-40A1-E13D
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person.identifier.ciencia-id6012-BDD5-083B
person.identifier.ciencia-id481A-FEE5-FC0A
person.identifier.ciencia-id621A-E288-BDF5
person.identifier.ciencia-id3A1D-FDA1-6D8E
person.identifier.orcid0000-0003-0193-4385
person.identifier.orcid0000-0002-9007-0082
person.identifier.orcid0000-0002-8886-545X
person.identifier.orcid0000-0002-9507-3311
person.identifier.orcid0000-0002-9676-1036
person.identifier.orcid0000-0003-4031-8999
person.identifier.orcid0000-0002-1628-7128
person.identifier.ridHTQ-8343-2023
person.identifier.scopus-author-id54789614100
person.identifier.scopus-author-id24485218100
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person.identifier.scopus-author-id6603861581
person.identifier.scopus-author-id55467624100
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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