Publication
Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
| dc.contributor.author | Bravo-Ferrada, B. M. | |
| dc.contributor.author | Gonçalves, S. | |
| dc.contributor.author | Semorile, L. | |
| dc.contributor.author | Santos, N. C. | |
| dc.contributor.author | Tymczyszyn, E. E. | |
| dc.contributor.author | Hollmann, A. | |
| dc.date.accessioned | 2016-05-04T15:32:41Z | |
| dc.date.available | 2016-05-04T15:32:41Z | |
| dc.date.issued | 2015 | |
| dc.description | © 2015 The Society for Applied Microbiology | pt_PT |
| dc.description.abstract | Aims: In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. Methods and Results: Damage on cell surface was studied by flow cytometry, zeta potential and atomic force microscopy, and cell survival was analysed by plate count. Results showed that beside cells acclimated at lower ethanol concentration (6% v/v) became more susceptible to drying than nonacclimated ones, after rehydration they maintain their increased ability to grow in a synthetic wine. Acclimation at a higher ethanol concentration (10% v/v) produces several damages on the cell surface losing its ability to grow in a synthetic wine. Conclusions: In this work, we showed for the first time that sublethal alterations on bacterial surface induced by a pre-acclimation with a low ethanol concentration (6%), upon a freeze-drying process, result in a better bacterial adaptation to the stress conditions of wine-like medium, as well as to the preservation process. Significance and Impact of the Study: Understanding the adaptation to ethanol of oenological strains and their effects on the preservation process has a strong impact on winemaking process and allows to define the most appropriate conditions to obtain malolactic starters cultures. | pt_PT |
| dc.description.sponsorship | This work has been funded by grants from Universidad Nacional de Quilmes (Programa Microbiología Molecular Básica y Aplicaciones Biotecnológicas, Argentina), Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT – MINCyT, Argentina; PICTO-UNQ 2006 N-36474 and PICT SU 2012 N° 2804), Comisión Nacional de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-BA, Argentina) and Fundação para a Ciência e Tecnologia – Ministério da Educação e Ciência (FCTMEC, Portugal). Bravo-Ferrada, B.M. has a fellowship from the Consejo Nacional de Investigaciones Científicasy Técnicas (CONICET). AH and EET are members of the Carrera del Investigador Científico y Tecnológico (CONICET, Argentina). LS is member of the Research Career of Comisión de Investigaciones Científicas de la Provinciade Buenos Aires (CIC-BA). | pt_PT |
| dc.identifier.citation | Journal of Applied Microbiology 118, 1409-1417 | pt_PT |
| dc.identifier.doi | 10.1111/jam.12796 | pt_PT |
| dc.identifier.issn | 1364-5072 | |
| dc.identifier.uri | http://hdl.handle.net/10451/23602 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Wiley | pt_PT |
| dc.relation.publisherversion | http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2672 | pt_PT |
| dc.subject | Atomic force microscopy | pt_PT |
| dc.subject | Freeze-drying | pt_PT |
| dc.subject | Lactobacillus plantarum | pt_PT |
| dc.subject | Wine | pt_PT |
| dc.subject | Zeta potential | pt_PT |
| dc.title | Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.title | Journal of Applied Microbiology | pt_PT |
| rcaap.rights | closedAccess | pt_PT |
| rcaap.type | article | pt_PT |