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COPa: Estudo clínico de análises de perfis de compostos orgânicos voláteis no ar exalado da respiração e na urina para diagnóstico e seguimento de doentes com cancro do pâncreas
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Resumo(s)
O objetivo deste estudo foi o da análise dos perfis de compostos orgânicos voláteis
presentes no ar exalado da respiração, e na urina, para deteção e triagem de
adenocarcinoma do pâncreas. Foram recrutados 21 voluntários na totalidade, tendo sido
divididos em dois grupos: Grupo de Controlo que contém os voluntários saudáveis e o
Grupo de Doentes que contém os voluntários diagnosticados com adenocarcinoma ductal
do pâncreas.
Foram recolhidas informações de todos os voluntários relevantes para o estudo,
como a idade, sexo, hábitos tabágicos e carga tabágica, exposições de risco e
comorbilidades.
A recolha das amostras de ar exalado foi realizada através da utilização de um
dispositivo fornecido pela Owlstone Medical, o ReCIVA Breath Biopsy, e de tubos de
dessorção térmica responsáveis pela retenção dos analitos. A urina dos voluntários
participantes foi manuseada numa fase posterior de modo que os compostos orgânicos
voláteis presentes fossem concentrados nos tubos de dessorção térmica. A análise química
do analito, no ar exalado e na urina, foi realizada através de Cromatografia em Fase
Gasosa acoplada a Espectrometria de Mobilidade Iónica de Campo Assimétrico,
permitindo a associação e comparação dos perfis correspondentes a cada grupo.
A análise estatística foi feita com recurso ao software Metaboanalyst, onde os dados
foram filtrados, normalizados e classificados de acordo com o seu perfil. Através do uso
de todos os dados obtidos das várias visitas dos doentes para tratamento, obteve-se uma
Especificidade de 95% e Sensibilidade de 96% para o ar exalado, e Especificidade de
78% e Sensibilidade de 62% para a urina.
The goal of this study was the analysis of the profiles of volatile organic compounds present in exhaled breath air and urine samples, for the detection and screening of pancreatic ductal adenocarcinoma. The recruitment consisted of a total of 21 volunteers, divided by two groups: Control Group containing healthy volunteers and Patient Group containing volunteers diagnosed with pancreatic ductal adenocarcinoma. Relevant information regarding all volunteers was gathered for the study, such as age, gender, smoking habits and smoking load, risk exposures and comorbidities. The collection of exhaled breath samples was performed by the Owlstone Medical device, ReCIVA Breath Biopsy, and thermic desorption tubes responsible for the analyte retention. The urine samples provided by the volunteer were handled afterwards, to concentrate its volatile organic compounds in the thermic desorption tubes. Chemical analysis of the analyte, present in the exhaled breath and urine, was performed through Gas Phase Chromatography coupled to Field Asymmetric Ion Mobility Spectrometry, allowing association and comparison of the corresponding profiles of each group. Statistical analysis was made with the Metaboanalyst software, where the data were filtered, normalized, and classified according to their profile. Using all the data obtained from the diverse patients visits for treatment, a Specificity of 95% and Sensitivity of 96% for exhaled breath were obtained, while for urine analysis a Specificity of 78% and Sensitivity of 62% were obtained instead.
The goal of this study was the analysis of the profiles of volatile organic compounds present in exhaled breath air and urine samples, for the detection and screening of pancreatic ductal adenocarcinoma. The recruitment consisted of a total of 21 volunteers, divided by two groups: Control Group containing healthy volunteers and Patient Group containing volunteers diagnosed with pancreatic ductal adenocarcinoma. Relevant information regarding all volunteers was gathered for the study, such as age, gender, smoking habits and smoking load, risk exposures and comorbidities. The collection of exhaled breath samples was performed by the Owlstone Medical device, ReCIVA Breath Biopsy, and thermic desorption tubes responsible for the analyte retention. The urine samples provided by the volunteer were handled afterwards, to concentrate its volatile organic compounds in the thermic desorption tubes. Chemical analysis of the analyte, present in the exhaled breath and urine, was performed through Gas Phase Chromatography coupled to Field Asymmetric Ion Mobility Spectrometry, allowing association and comparison of the corresponding profiles of each group. Statistical analysis was made with the Metaboanalyst software, where the data were filtered, normalized, and classified according to their profile. Using all the data obtained from the diverse patients visits for treatment, a Specificity of 95% and Sensitivity of 96% for exhaled breath were obtained, while for urine analysis a Specificity of 78% and Sensitivity of 62% were obtained instead.
Descrição
Tese de Mestrado, Química, 2023, Universidade de Lisboa, Faculdade de Ciências
Palavras-chave
Compostos orgânicos voláteis Adenocarcinoma do pâncreas Ar exalado Urina Teses de mestrado - 2023