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Unravelling the hidden inter and intra-varietal diversity of durum wheat commercial varieties used in Portugal
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Orientador(es)
Resumo(s)
Assessing durum wheat genomic diversity is crucial in a changing environmental particularly in the
Mediterranean region where it is largely used to produce pasta. Durum wheat varieties cultivated in
Portugal and previously assessed regarding thermotolerance ability were screened for the variability
of coding sequences associated with technological traits and repetitive sequences. As expected, reduced
variability was observed regarding low molecular weight glutenin subunits (LMW-GS) but a
specific LMW-GS allelic form associated with improved pasta-making characteristics was absent in
one variety. Contrastingly, molecular markers targeting repetitive elements like microsatellites and
retrotransposons – Inter Simple Sequence Repeat (ISSR) and Inter Retrotransposons Amplified
Polymorphism (IRAP) – disclosed significant inter and intra-varietal diversity. This high level of polymorphism
was revealed by the 20 distinct ISSR/IRAP concatenated profiles observed among the 23
individuals analysed. Interestingly, median joining networks and PCoA analysis grouped individuals
of the same variety and clustered varieties accordingly with geographical origin. Globally, this work
demonstrates that durum wheat breeding strategies induced selection pressure for some relevant
coding sequences while maintaining high levels of genomic variability in non-coding regions enriched
in repetitive sequences
Descrição
Short Communication
Palavras-chave
genetic diversity glutenins microsatellites retrotransposons
Contexto Educativo
Citação
Plant Genetic Resources: Characterization and Utilization; 1–4
Editora
NIAB