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First interspecific genetic linkage map for Castanea sativa x Castanea crenata revealed QTLs for resistance to Phytophthora cinnamomi
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Orientador(es)
Resumo(s)
The Japanese chestnut (Castanea crenata) carries resistance to Phytophthora cinnamomi,
the destructive and widespread oomycete causing ink disease. The European chestnut
(Castanea sativa), carrying little to no disease resistance, is currently threatened by the
presence of the oomycete pathogen in forests, orchards and nurseries. Determining the
genetic basis of P. cinnamomi resistance, for further selection of molecular markers and
candidate genes, is a prominent issue for implementation of marker assisted selection in the
breeding programs for resistance. In this study, the first interspecific genetic linkage map of
C. sativa x C. crenata allowed the detection of QTLs for P. cinnamomi resistance. The
genetic map was constructed using two independent, control-cross mapping populations.
Chestnut populations were genotyped using 452 microsatellite and single nucleotide polymorphism
molecular markers derived from the available chestnut transcriptomes. The consensus
genetic map spans 498,9 cM and contains 217 markers mapped with an average
interval of 2.3 cM. For QTL analyses, the progression rate of P. cinnamomi lesions in
excised shoots inoculated was used as the phenotypic metric. Using non-parametric and
composite interval mapping approaches, two QTLs were identified for ink disease resistance,
distributed in two linkage groups: E and K. The presence of QTLs located in linkage
group E regarding P. cinnamomi resistance is consistent with a previous preliminary study
developed in American x Chinese chestnut populations, suggesting the presence of common
P. cinnamomi defense mechanisms across species. Results presented here extend the genomic resources of Castanea genus providing potential tools to assist the ongoing
and future chestnut breeding programs
Descrição
Research Article
Palavras-chave
Castanea sativa Castanea crenata Phytophthora cinnamomi
Contexto Educativo
Citação
Santos C, Nelson CD, Zhebentyayeva T, Machado H, Gomes-Laranjo J, Costa RL (2017) First interspecific genetic linkage map for Castanea sativa x Castanea crenata revealed QTLs for resistance to Phytophthora cinnamomi. PLoS ONE 12(9): e0184381
Editora
Yong Pyo Lim (ed.)