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Culture media and sampling collection method for Aspergillus spp. assessment : tackling the gap between recommendations and the scientific evidence

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dc.contributor.authorViegas, Carla
dc.contributor.authorDias, Marta
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorSabino, Raquel Filipa Pinheiro
dc.date.accessioned2021-01-07T12:40:01Z
dc.date.available2021-01-07T12:40:01Z
dc.date.issued2020
dc.descriptionCopyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.pt_PT
dc.description.abstractCulturing is still the most widely used method for determining fungal growth. Thus, is important to identify the most suitable culture media to assess Aspergillus spp. The aim of this study was to analyze data obtained from previous studies, aiming at identifying the most suitable culture media (malt extract agar (MEA) or dichloran-glycerol agar (DG18) to assess Aspergillus spp. isolation and growth. This study was conducted by using environmental samples (n = 1153). Most of the active sampling methods (air samples) were impacted directly onto both culture media. As for passive sampling methods, fungi were extracted from environmental matrices inoculated onto both media. Overall, total Aspergillus counts were higher in MEA (n = 617, 53.5%) than in DG18 (n = 536, 46.5%). Regarding Aspergillus sections, significant associations were detected with the media (χ2 (7) = 241.118, p < 0.001), the sampling approach (p < 0.001, 95% CI = (0.3 × 10−4), and the indoor environment (p < 0.001, 95% CI = (0.3 × 10−4)). As such, sampling approach and the culture media should be accurately selected when dealing with Aspergillus spp. exposure assessment.pt_PT
dc.description.sponsorshipThis work was supported by FCT—Fundação para Ciência e Tecnologia for funding the project EXPOsE—Establishing protocols to assess occupational exposure to microbiota in clinical settings (02/SAICT/2016—Project n° 23222) and Instituto Politécnico de Lisboa, Lisbon, Portugal for funding the Project “Waste Workers’ Exposure to Bio-burden through Filtering Respiratory Protective Devices” (IPL/2018/WasteFRPD_ESTeSL) and the Project “Occupational exposure of ambulance drivers to bioburden” (IPL/2020/BIO-AmbuDrivers_ESTeSL). H&TRC authors gratefully acknowledge the FCT/MCTES national support through the UIDB/05608/2020 and UIDP/05608/2020.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAtmosphere 2021, 12(1), 23pt_PT
dc.identifier.doi10.3390/atmos12010023pt_PT
dc.identifier.eissn2073-4433
dc.identifier.urihttp://hdl.handle.net/10451/45689
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation02/SAICT/2016pt_PT
dc.relationHealth and Technology Research Center
dc.relationHealth and Technology Research Center
dc.relation.publisherversionhttps://www.mdpi.com/journal/atmospherept_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectExposurept_PT
dc.subjectMEApt_PT
dc.subjectDG18pt_PT
dc.subjectSamplingpt_PT
dc.subjectIndoorpt_PT
dc.subjectAspergillus spp.pt_PT
dc.titleCulture media and sampling collection method for Aspergillus spp. assessment : tackling the gap between recommendations and the scientific evidencept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth and Technology Research Center
oaire.awardTitleHealth and Technology Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05608%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F05608%2F2020/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage23pt_PT
oaire.citation.titleAtmospherept_PT
oaire.citation.volume12pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameViegas
person.familyNameSabino
person.givenNameCarla
person.givenNameRaquel Filipa Pinheiro
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0001-6585-7775
person.identifier.scopus-author-id35796067600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication349fba83-0a3e-4c43-8658-b76cc75f673c
relation.isAuthorOfPublication47ebbeef-3e52-47b2-aa6f-def8dd415927
relation.isAuthorOfPublication.latestForDiscovery349fba83-0a3e-4c43-8658-b76cc75f673c
relation.isProjectOfPublication9573e9cd-0c34-4e6b-887b-12ba6805a472
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